A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}crb07207 Insertion

General Information
Symbol Dmel\P{PZ}crb07207 Species D. melanogaster
Name FlyBase ID FBti0005582
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) crb, Ecol\lacZ Viability / fertility
Causes allele(s) crb07207, Ecol\lacZcrb-07207 Stock availability 1 publicly available
LINE ID l(3)07207
Genomic Location
Chromosomal location 3R ( 95F10 ) Sequence location 3R:20,120,747..20,120,747 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0143132 FBti0040161 FBti0105737 FBti0132320 FBti0035134 FBti0007910 FBti0005582 FBti0074926 FBti0009901 FBti0029711 FBti0025610 FBti0023157 FBti0038739 FBti0052672 FBti0054704 FBti0110662 FBti0043460 FBti0048681
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:20,120,747..20,120,747 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
95F10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
95F11-95F12 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073342
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
crb
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
hide Alleles and Phenotypes
Causes alleles
crb07207
(BDGP Project Members, 1994-1999, Read et al., 2005, Spradling et al., 1999)
Ecol\lacZcrb-07207
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Lethality
References
lethal | larval stage | recessive
(Perrimon et al., 1996)
lethal | recessive
(Spradling et al., 1999)
Sterility
References
female sterile
(Perrimon et al., 1996)
hide Phenotype Manifest In
egg | germline clone
(Perrimon et al., 1996)
embryonic/first instar larval cuticle | ventral
(Perrimon et al., 1996)
ovariole | germline clone
(Perrimon et al., 1996)
hide Detailed Description
Statement
Reference
Homozygous embryos exhibit absence of ventral hypoderm. Germline clones produce abnormal eggs due to abnormal oogenesis: small collapsed eggs.
(Perrimon et al., 1996)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
Malpighian tubule | restricted  
(Meister and Braun, 1995.10)
embryonic/larval foregut  
(Meister and Braun, 1995.10)
embryonic/larval trachea  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
embryonic/larval hindgut  
(Meister and Braun, 1995.10)
adult stage  
ocellus  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)07207
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11718
ry506 P{PZ}crb07207/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1718
(Meister and Braun, 1995.10)
P{PZ}crb07207
(FlyBase, 1992-, )
P{PZ}l(3)0720707207
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0720707207
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000636
hide References ( 11 )
Research paper
Read et al., 2005, Genetics 171(3): 1057--1081
A Drosophila model of multiple endocrine neoplasia type 2. [FBrf0190765]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Perrimon et al., 1996, Genetics 144(4): 1681--1692
Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]