A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}eIF-4E07238 Insertion

General Information
Symbol Dmel\P{PZ}eIF-4E07238 Species D. melanogaster
Name FlyBase ID FBti0005584
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, eIF-4E Viability / fertility
Causes allele(s) Ecol\lacZeIF-4E-07238, eIF-4E07238 Stock availability 1 publicly available
LINE ID l(3)07238
Genomic Location
Chromosomal location 3L ( 67B4 ) Sequence location 3L:9,395,069..9,395,069 [+]
Map ( GBrowse ) detailed view FBti0020077 FBti0125593_1 FBti0125593_2 FBti0056798 FBti0024758 FBti0103402 FBti0024902 FBti0106739 FBti0047271 FBti0011519 FBti0052539 FBti0016443 FBti0110145 FBti0029960 FBti0110144 FBti0029549 FBti0025612 FBti0005584 FBti0099892 FBti0055204 FBti0071191 FBti0050960 FBti0048110 FBti0125594 FBti0070559 FBti0044702 FBti0041299 FBti0111307 FBti0041235 FBti0075978 FBti0124451
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:9,395,069..9,395,069 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
67B4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
67B1-67B2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034073
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Harvie et al., 1998)
eIF-4E
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZeIF-4E-07238
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10, Harvie et al., 1998)
eIF-4E07238
(BDGP Project Members, 1994-1999, Andrade and Cook, 2005.7.22, Lachance et al., 2002, Harvie et al., 1998, Spradling et al., 1999)
Lethality
References
lethal
(Ottone et al., 2011)
lethal | larval stage | recessive
(Lachance et al., 2002)
lethal | recessive
(Harvie et al., 1998, Spradling et al., 1999)
viable | reduced
(Lachance et al., 2002)
Sterility
References
hide Phenotype Manifest In
wing
(Lachance et al., 2002)
hide Detailed Description
Statement
Reference
The pupal lethality observed in park[P23]/park[P23] mutant animals is suppressed in a eIF-4E[07238]/+ background. Pupal lethality is restored in Thor[Δ1034-2617]/Thor[Δ1034-2617]; park[P23], eIF-4E[07238]/park[P23] animals. The number of mature oocytes oviposited from park[P23], eIF-4E[07238]/park[P23] mutant females mated to wild-type males is 42.8 oocytes/mother, and the rate of larval hatching is 40.5%, much improved compared to park[P23] homozygous mutant females. eIF-4E[07238]/+ significantly abrogates the oogenesis defects in park[P23]/park[P23] mutant females. Most egg chambers appear normal in these animals, and the reduced size of park[P23]/park[P23] ovaries is rescued in a eIF-4E[07238]/+ background. Oogenesis defects are not restored in Thor[Δ1034-2617]/Thor[Δ1034-2617]; park[P23], eIF-4E[07238]/park[P23] mutant animals.
(Ottone et al., 2011)
The increased sensitivity to paraquat that is seen in flies expressing Lrrk[I1915T.Scer\UAS] under the control of Scer\GAL4[da.G32] is suppressed to wild-type levels of sensitivity if the flies are also heterozygous for eIF-4E[07238].
(Imai et al., 2008)
Development is arrested in the second larval instar, although the larvae survive for up to 10 days. Developmental delay with eIF-4ESer251Ala.t8.9 is 1 to 2 days.
(Lachance et al., 2002)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Harvie et al., 1998, Meister and Braun, 1995.10)
embryonic/larval optic lobe  
(Harvie et al., 1998)
ventral nerve cord  
(Harvie et al., 1998)
ring gland  
(Harvie et al., 1998)
adult stage  
adult brain | restricted  
(Vosshall, 1998.8.10)
adult central complex  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
In the adult, low level of expression in the central brain.
(Vosshall, 1998.8.10)
In wandering third instar larva, expression in imaginal discs (which discs not specified).
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)07238
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11720
P{PZ}eIF-4E07238 ry506/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3L:9375916-9375917 confirmed by FlyBase alignment of dbGSS accession AQ034073 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1720
(Meister and Braun, 1995.10)
P{PZ}eIF-4E07238
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}l(3)0723807238
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0723807238
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000638
hide References ( 14 )
Research paper
Ottone et al., 2011, Gene 470(1-2): 12--19
Diminution of eIF4E activity suppresses parkin mutant phenotypes. [FBrf0212264]
Imai et al., 2008, EMBO J. 27(18): 2432--2443
Phosphorylation of 4E-BP by LRRK2 affects the maintenance of dopaminergic neurons in Drosophila. [FBrf0207127]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Lachance et al., 2002, Molec. Cell. Biol. 22(6): 1656--1663
Phosphorylation of Eukaryotic Translation Initiation Factor 4E Is Critical for Growth. [FBrf0145121]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Harvie et al., 1998, Genetics 149(1): 217--231
Genes expressed in the ring gland, the major endocrine organ of Drosophila melanogaster. [FBrf0102844]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Andrade and Cook, 2005.7.22, Isolation and characterization of Df(3L)BSC113.
Isolation and characterization of Df(3L)BSC113. [FBrf0188655]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]