A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Hel89B08724 Insertion

General Information
Symbol Dmel\P{PZ}Hel89B08724 Species D. melanogaster
Name FlyBase ID FBti0005592
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Hel89B, ms(3)08724 Viability / fertility viable
Causes allele(s) Ecol\lacZ08724, Hel89B08724 Stock availability 1 publicly available
LINE ID l(3)08724 ms(3)08724
Genomic Location
Chromosomal location 3R ( 89A11 ) Sequence location 3R:11,801,757..11,801,757 [+]
Map ( GBrowse ) detailed view FBti0025628 FBti0103435 FBti0103731 FBti0105952 FBti0108841 FBti0024852 FBti0074930 FBti0033577 FBti0024618 FBti0008004 FBti0054235 FBti0107480 FBti0072668 FBti0034389 FBti0103262 FBti0109522 FBti0070549 FBti0106409 FBti0047842 FBti0005592 FBti0074988 FBti0005046
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:11,801,757..11,801,757 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
89A11 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
89B1--89B3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
89B1-89B3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026437
(FlyBase, 1992-)
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hel89B
(Yagi and Ip, 2005)
ms(3)08724
(Kennison, 1995.6.21)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZ08724
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hel89B08724
(BDGP Project Members, 1994-1999, Kennison, 1995.6.21, Spradling et al., 1999, Yagi and Ip, 2005)
Lethality
References
semi-lethal
(Yagi and Ip, 2005)
viable
(Kennison, 1995.6.21)
Sterility
References
male sterile | recessive
(Spradling et al., 1999)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
42% of Hel89B[08724] mutant larvae hatch as adults. After injury or septic injury, the survival rate of Hel89B[08724] mutant larvae drops from 89% to 73% before pupariation, and survival drops from 42% to 14% before adulthood.
(Yagi and Ip, 2005)
No record of any lethality associated with this stock.
(Spradling, 1999.12.9)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage  
medulla  
(Vosshall, 1998.8.10)
ocellus  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)08724
(Gene Disruption Project members, 2001-)
ms(3)08724
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11732
ry506 P{PZ}Hel89B08724, l(3)0872408724/TM3, ryRK Sb1 Ser1
hide Linkouts
hide Comments
Location 3R:11801757-11801758 confirmed by FlyBase alignment of dbGSS accession AQ026437 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
The lethality of the chromosome (caused by l(3)0872408724) is separable from the P{PZ}ms(3)0872408724 insertion, which causes male sterility.
(Kennison, 1995.6.21)
Separable from putative lethal induced on same chromosome; see l(3)0872408724.
(Kennison, 1995.6.21)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1732
(Yagi and Ip, 2005)
P{PZ}08724
(FlyBase, 1992-)
P{PZ}Hel89B08724
 
P{PZ}l(3)0872408724
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}ms(3)0872408724
(FlyBase, 2005, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0872408724
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000646
  • FBti0010314
hide References ( 11 )
Research paper
Yagi and Ip, 2005, EMBO Rpts 6(11): 1088--1094
Helicase89B is a Mot1p/BTAF1 homologue that mediates an antimicrobial response in Drosophila. [FBrf0190377]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Spradling, 1999.12.9, Helping FlyBase with P-element paper.
Helping FlyBase with P-element paper. [FBrf0125024]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Kennison, 1995.6.21, Complementation data for assorted P{} insertions in the Bloomington collection.
Complementation data for assorted P{} insertions in the Bloomington collection. [FBrf0082216]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]