A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}Glut4EF10477 Insertion

General Information
Symbol Dmel\P{PZ}Glut4EF10477 Species D. melanogaster
Name FlyBase ID FBti0005602
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Glut4EF Viability / fertility
Causes allele(s) Ecol\lacZstretch-10477, Glut4EF10477 Stock availability none publicly available
LINE ID l(3)10477
Genomic Location
Chromosomal location 3R ( 85E14-85F5 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
85E14-85F5 ( near gene of known cytology )
Cytological location
(reported)
85F1-85F2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Glut4EF
(Terman et al., 2002, BDGP Project Members, 1994-1999, Yazdani et al., 2008)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZstretch-10477
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Glut4EF10477
(Terman et al., 2002, Yazdani et al., 2008)
Lethality
References
partially lethal - majority die
 
viable
(Yazdani et al., 2008)
Sterility
References
hide Phenotype Manifest In
wing
(Terman et al., 2002, BDGP Project Members, 1994-1999, Yazdani et al., 2008)
hide Detailed Description
Statement
Reference
Homozygotes and Glut4EF[10477]/Df(3R)by62 adults hold their wings out at an angle.
(Yazdani et al., 2008)
Mutant flies hold their wings out at a 45[o] angle from their bodies.
(Terman et al., 2002)
Wings held out at an angle.
(BDGP Project Members, 1994-1999)
visible wings held out at angle
 
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage  
third segment of antenna  
(Vosshall, 1998.8.10)
adult brain | restricted  
(Vosshall, 1998.8.10)
adult central complex  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)10477
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1746
(Meister and Braun, 1995.10)
P{PZ}Glut4EF10477
 
P{PZ}l(3)10477
(Ostrowski et al., 2002)
P{PZ}l(3)1047710477
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}stretch10477
(Yazdani et al., 2008, FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)1047710477
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000658
hide References ( 12 )
Research paper
Yazdani et al., 2008, Genetics 178(2): 919--929
The glucose transporter (GLUT4) enhancer factor is required for normal wing positioning in Drosophila. [FBrf0204343]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Ostrowski et al., 2002, Genetics 161(1): 171--182
Genetic control of cuticle formation during embryonic development of Drosophila melanogaster. [FBrf0149011]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Supplementary material
Terman et al., 2002, Cell 109(7):
MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. [FBrf0150847]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]