Dmel\P{PZ}vn¹⁰⁵⁶⁷ Insertion

General Information
Symbol	Dmel\P{PZ}vn¹⁰⁵⁶⁷	Species	D. melanogaster
Name		FlyBase ID	FBti0005605
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, vn	Viability / fertility
Causes allele(s)	Ecol\lacZ^vn-10567, vn¹⁰⁵⁶⁷	Stock availability	1 publicly available
LINE ID	l(3)10567
Genomic Location
Chromosomal location	3L ( 64F2 )	Sequence location	3L:5,837,369..5,837,369 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:5,837,369..5,837,369 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	64F2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	64F1-64F3 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034078 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Yarnitzky et al., 1997, Sen et al., 2004, Kiger et al., 2000, Vosshall, 1998.8.10, Jiang and Edgar, 2009, Buchon et al., 2010, Takemura and Adachi-Yamada, 2011) vn (BDGP Project Members, 1994-1999, Jiang and Edgar, 2009)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^vn-10567 (Yarnitzky et al., 1997, Sen et al., 2004, Kiger et al., 2000, Vosshall, 1998.8.10, Jiang and Edgar, 2009, Buchon et al., 2010, Takemura and Adachi-Yamada, 2011) vn¹⁰⁵⁶⁷ (BDGP Project Members, 1994-1999, Spradling et al., 1999, Urban et al., 2004, Yarnitzky et al., 1998, Yarnitzky et al., 1997, Eggert et al., 1998, Jiang and Edgar, 2009)

Lethality References lethal \| pharate adult stage (Jiang and Edgar, 2009) lethal \| recessive (Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	abdominal 2 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 3 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 4 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 5 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 6 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) abdominal 7 dorsal acute muscle 1 \| precursor (Yarnitzky et al., 1998) adult midgut precursor cell \| larval stage (Jiang and Edgar, 2009) adult midgut precursor cell \| late third instar larval stage (Jiang and Edgar, 2009) dorsal oblique muscle (Yarnitzky et al., 1997) embryonic/larval midgut \| embryonic stage (Szuts et al., 1998) embryonic/larval somatic muscle (Yarnitzky et al., 1998) gastric caecum (Szuts et al., 1998) lch5 ligament attachment cell (Inbal et al., 2004) midgut constriction 1 (Szuts et al., 1998) midgut constriction 2 (Szuts et al., 1998) midgut constriction 3 (Szuts et al., 1998) ventral longitudinal muscle (Yarnitzky et al., 1997) ventral oblique muscle (Yarnitzky et al., 1997)
Detailed Description
Statement Reference Expression of vn[Scer\UAS.cSa] in larval enterocytes driven by Scer\GAL4[Myo31DF-NP0001] not only rescues the adult midgut progenitor cell phenotype of vn[10567] mutants, but it also causes ectopic AMP cell proliferation. Expression of vn[Scer\UAS.cSa] in adult midgut progenitor cells (AMPs) rescues the AMP cell proliferation phenotype of vn[10567] mutants. Expression of vn[Scer\UAS.cSa] in visceral muscle driven by Scer\GAL4[how-24B] rescues the adult midgut progenitor cell phenotype of vn[10567] mutants. (Jiang and Edgar, 2009) Whereas wild-type adult midgut progenitor cells (AMPs) form many large clusters, few AMP clusters are found in late larval vn[10567] and vn[10567]/vn[γ7] mutant midguts. Most vn[10567]/vn[γ7] mutants die as pharate adults. Lineage analysis of marked clones reveals that vn[10567]/vn[γ7] mutants display a failure of proliferation of AMPs during early larval stages, although the number of AMPs generated initially during embryogenesis is not different between mutants and wild-type controls. The size of the few remaining AMP clusters in vn[10567]/vn[γ7] mutants is relatively normal, suggesting that the late phase of AMP proliferation is largely unaffected in these mutants. Expression of vn[Scer\UAS.cSa] in larval enterocytes driven by Scer\GAL4[Myo31DF-NP0001] not only rescues the adult midgut progenitor cell phenotype of vn[10567] mutants, but it also causes ectopic AMP cell proliferation. (Jiang and Edgar, 2009) Although extra wing vein material is induced in Gug¹⁴⁹⁶⁷ clones, wing vein differentiation is blocked in vn¹⁰⁵⁶⁷ rho^PΔ5 Gug¹⁴⁹⁶⁷ triple mutant clones. (Charroux et al., 2006) Late stage vn¹⁰⁵⁶⁷/Df(3L)v65c embryos lack lch5 ligament attachment cells. (Inbal et al., 2004) 2/3 of vn¹⁰⁵⁶⁷; rho^unspecified double homozygous embryo cuticles show a general loss of cuticle integrity. Of the remaining 1/3 of cuticles the percentage with >2 denticle belt fusions is increased from <10% for rho^unspecified to >30%. (Note, while the authors do not name an rho allele for this analysis, they do claim to have used a null allele.) (Urban et al., 2004) Border cells still migrate dorsally when all dorsal follicle cells are mutant for vn¹⁰⁵⁶⁷ in females containing homozygous clones. (Duchek and Rorth, 2001) Shows very mild midgut phenotype. vn¹⁰⁵⁶⁷/Df(3L)γ3 causes lack of midgut constrictions and failure of elongation of gastric caeca. (Szuts et al., 1998) The loss of the DA1 muscle precursor in vn¹⁰⁵⁶⁷ embryos is enhanced if they are also carrying Egfr^DN.Scer\UAS expressed under the control of Scer\GAL4^how-24B. The loss of the DA1 muscle precursor seen in vn¹⁰⁵⁶⁷ embryos is dominantly enhanced by spi³. (Yarnitzky et al., 1998) A significant number of somatic muscle fibres show multiple, mis-guided membrane extensions at their leading edges. The DA1 muscle precursor is sometimes missing. (Yarnitzky et al., 1998) Abnormal muscle phenotype. Myotubes of mutant embryos are elongated and continuously send filopodia in random directions. (Yarnitzky et al., 1997)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) Malpighian tubule \| restricted (Meister and Braun, 1995.10) embryonic/larval hindgut (Meister and Braun, 1995.10) pupal stage P5(ii) -- pupal stage P6 antennal disc \| restricted (Sen et al., 2004) Comment:reference states 18-20 hr APF
Additional Information
Statement Reference Expression is observed in the region of the pupal antenna that is occupied mainly by coeloconic sensilla. Expression is not observed in glial cells. (Sen et al., 2004)

Marker for	pupal antenna disc (Sen et al., 2004)
Reflects expression of	vn (Sen et al., 2004)
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)10567 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11749 cn¹; P{PZ}vn¹⁰⁵⁶⁷ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Location 3L:5818216-5818217 confirmed by FlyBase alignment of dbGSS accession AQ034078 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}l(3)10567¹⁰⁵⁶⁷ (FlyBase, 1992-) P{PZ}vn 10567 (Kiger et al., 2000) P{PZ}vn¹⁰⁵⁶⁷ (FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-, ) P{ry^+t7.2=PZ}l(3)10567¹⁰⁵⁶⁷ (FlyBase, 1992-) vein-lacZ (Sen et al., 2004, Buchon et al., 2010) vnlacZ (Jiang and Edgar, 2009) vn-lacZ¹⁰⁵⁶⁷ (Takemura and Adachi-Yamada, 2011) vnlacZ^P1749 (Jiang and Edgar, 2009)
Secondary FlyBase IDs
	FBti0000661
References ( 23 )

Generate a list of	All references relating to this insertion ( 23 )

List References by type	Research paper ( 16 ) Personal communication to FlyBase ( 4 ) FlyBase analysis ( 3 )
Recent research papers ( 1 )
	Takemura and Adachi-Yamada, 2011, Dev. Biol. 357(2): 336--346 Cell death and selective adhesion reorganize the dorsoventral boundary for zigzag patterning of Drosophila wing margin hairs. [FBrf0214791] Show all research papers ...

Dmel\P{PZ}vn10567 Insertion

Dmel\P{PZ}vn¹⁰⁵⁶⁷ Insertion