Dmel\P{lacW}th^j5C8 Insertion

General Information
Symbol	Dmel\P{lacW}th^j5C8	Species	D. melanogaster
Name		FlyBase ID	FBti0005620
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, th	Viability / fertility
Causes allele(s)	Ecol\lacZ^th-j5C8, th^j5C8	Stock availability	2 publicly available
LINE ID	l(3)j5C8
Genomic Location
Chromosomal location	3L ( 72D1 )	Sequence location	3L:16,039,894..16,039,894 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:16,039,894..16,039,894 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	72D1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	72D1-72D2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034091 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Silva, 2006, Willecke et al., 2006, Zhang et al., 2008, Oh and Irvine, 2008, Braun et al., 1997, Huang et al., 2005, Silva et al., 2006, Bennett and Harvey, 2006, Geisbrecht and Montell, 2004, Ryoo et al., 2002, Jia et al., 2003, Lai et al., 2005, Zhao et al., 2007, Sun et al., 2011, Cho et al., 2006, Reddy and Irvine, 2011, Oh and Irvine, 2011, Hamaratoglu et al., 2006, Das Thakur et al., 2010, Yu et al., 2010, Delanoue et al., 2004, Betz et al., 2008, Ling et al., 2010, Zeng et al., 2010, Grusche et al., 2011, Yan et al., 2011, Rauskolb et al., 2011, Cordero and Cagan, 2010, Gilbert et al., 2011, Wu et al., 2008, Chen and Verheyen, 2012) th (Hay et al., 1995, Chen et al., 2008, Cooper and Kennison, 2011, Verghese et al., 2012)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^th-j5C8 (Silva, 2006, Braun et al., 1997, Huang et al., 2005, Silva et al., 2006, Bennett and Harvey, 2006, Geisbrecht and Montell, 2004, Ryoo et al., 2002, Jia et al., 2003, Willecke et al., 2006, Zhang et al., 2008, Lai et al., 2005, Oh and Irvine, 2008, Zhao et al., 2007, Sun et al., 2011, Cho et al., 2006, Reddy and Irvine, 2011, Oh and Irvine, 2011, Hamaratoglu et al., 2006, Das Thakur et al., 2010, Yu et al., 2010, Delanoue et al., 2004, Betz et al., 2008, Ling et al., 2010, Zeng et al., 2010, Grusche et al., 2011, Yan et al., 2011, Rauskolb et al., 2011, Cordero and Cagan, 2010, Gilbert et al., 2011, Wu et al., 2008, Chen and Verheyen, 2012) th^j5C8 (Hay et al., 1995, BDGP Project Members, 1994-1999, Huang et al., 2005, Geisbrecht and Montell, 2004, Lai et al., 2005, Braun et al., 1997, Wu et al., 2003, Spradling et al., 1999, Chen et al., 2008, Cooper and Kennison, 2011, Verghese et al., 2012)

Lethality References lethal \| recessive (Spradling et al., 1999, Hay et al., 1995)
Sterility References
Phenotype Manifest In
	border follicle cell \| somatic clone (Geisbrecht and Montell, 2004) follicle cell \| somatic clone (Geisbrecht and Montell, 2004)
Detailed Description
Statement Reference Homozygous follicle cells clones result in border follicle cell migration defects. In large homozygous clones in the follicle epithelium, multinucleate cells are seen. Mutant outer follicle cell clones do not show TUNEL labeling, even 10 days after clone induction. (Geisbrecht and Montell, 2004) The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by th^j5C8. (Shulman and Feany, 2003)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference late third instar larval stage wing disc \| ubiquitous (Ryoo et al., 2002) Comment:Expression strongest at D/V boundary wing disc \| dorsal/ventral compartment boundary (Ryoo et al., 2002)
Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j5C8 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12093 y¹ w^*; P{lacW}th^j5C8/TM3, Sb¹
Kyoto	111396 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}th^j5C8 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:16012053-16012054 confirmed by FlyBase alignment of dbGSS accession AQ034091 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	diap1^j5C8 (Verghese et al., 2012) diap1-lacZ (Lai et al., 2005, Zhao et al., 2007, Oh and Irvine, 2011, Yu et al., 2010, Betz et al., 2008, Ling et al., 2010, Grusche et al., 2011, Yan et al., 2011, Gilbert et al., 2011, Wu et al., 2008, Sun et al., 2011, Cho et al., 2006) Diap1-lacZ (Silva et al., 2006, Silva, 2006, Chen and Verheyen, 2012) DIAP1-lacZ (Bennett and Harvey, 2006, Willecke et al., 2006, Das Thakur et al., 2010) DIAP1--lacZ (Hamaratoglu et al., 2006) diap-lacZ (Jia et al., 2003, Silva et al., 2006, Silva, 2006) Diap-LacZ (Zeng et al., 2010) P{lacW}th^j5C8 (FlyBase, 2005, Hay et al., 1995, FlyBase, 1992-, Chen et al., 2008, ) P{w^+mC=lacW}th^j5C8 (FlyBase, 1992-) th^i5c8 (Delanoue et al., 2004) th^j5c8 (Grusche et al., 2011) th^j5c8 diap1-lacZ (Huang et al., 2005) th^l(3)j5C8 lacZ (Geisbrecht and Montell, 2004) th-lacZ (Oh and Irvine, 2008, Rauskolb et al., 2011, Reddy and Irvine, 2011) thread-lacZ (Oh and Irvine, 2011, Cho et al., 2006) th-Z (Gilbert et al., 2011)
Secondary FlyBase IDs
	FBti0001224
References ( 45 )

Generate a list of	All references relating to this insertion ( 45 )

List References by type	Research paper ( 39 ) Supplementary material ( 1 ) Personal communication to FlyBase ( 2 ) FlyBase analysis ( 3 )
Recent research papers ( 10 )
	Chen and Verheyen, 2012, Curr. Biol. 22(17): 1582--1586 Homeodomain-interacting protein kinase regulates yorkie activity to promote tissue growth. [FBrf0219448] Verghese et al., 2012, Cell Death Differ. 19(10): 1664--1676 Hippo signalling controls Dronc activity to regulate organ size in Drosophila. [FBrf0219371] Cooper and Kennison, 2011, PLoS ONE 6(8): e23509 Molecular Genetic Analyses of Polytene Chromosome Region 72A-D in Drosophila melanogaster Reveal a Gene Desert in 72D. [FBrf0214748] Gilbert et al., 2011, Dev. Cell 20(5): 700--712 A Screen for Conditional Growth Suppressor Genes Identifies the Drosophila Homolog of HD-PTP as a Regulator of the Oncoprotein Yorkie. [FBrf0213686] Grusche et al., 2011, Dev. Biol. 350(2): 255--266 The Salvador/Warts/Hippo pathway controls regenerative tissue growth in Drosophila melanogaster. [FBrf0212888] Oh and Irvine, 2011, Dev. Cell 20(1): 109--122 Cooperative Regulation of Growth by Yorkie and Mad through bantam. [FBrf0212763] Rauskolb et al., 2011, PLoS Biol. 9(6): e1000624 Zyxin links fat signaling to the hippo pathway. [FBrf0213900] Reddy and Irvine, 2011, Development 138(23): 5201--5212 Regulation of Drosophila glial cell proliferation by Merlin-Hippo signaling. [FBrf0216584] Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Yan et al., 2011, Development 138(9): 1697--1703 Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. [FBrf0213488] Show all research papers ...

Dmel\P{lacW}thj5C8 Insertion

Dmel\P{lacW}th^j5C8 Insertion