A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{hsneo}l(3)neo11 Insertion

General Information
Symbol Dmel\P{hsneo}l(3)neo11 Species D. melanogaster
Name FlyBase ID FBti0005621
Feature type transposable_element_insertion_site
Description
Inserted element P{hsneo} Expression data
Affected gene(s) l(3)neo1 Viability / fertility
Causes allele(s) l(3)neo11 Stock availability 1 publicly available
LINE ID l(3)neo1
Genomic Location
Chromosomal location 3L ( 61C7 ) Sequence location 3L:639,303..639,303 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0057258 FBti0044779 FBti0055981 FBti0065917 FBti0039307 FBti0028888 FBti0103798 FBti0025088 FBti0011383 FBti0039574 FBti0111922 FBti0151887 FBti0029104 FBti0029601 FBti0005621 FBti0025587 FBti0055922 FBti0030254 FBti0072731 FBti0112156 FBti0055907 FBti0107105 FBti0109850 FBti0045599 FBti0028347 FBti0036557 FBti0068599 FBti0069008 FBti0107185 FBti0029125 FBti0130042 FBti0130040 FBti0009917 FBti0102365 FBti0037203 FBti0106056 FBti0108914 FBti0034887 FBti0005549 FBti0102398 FBti0029241 FBti0104974 FBti0105853 FBti0037022 FBti0048079 FBti0050097 FBti0066056 FBti0065026 FBti0065774 FBti0065233 FBti0044051 FBti0044052 FBti0044929 FBti0048445 FBti0125455_2 FBti0125455_1 FBti0044274 FBti0147199 FBti0043882 FBti0021740 FBti0076049
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{hsneo}; most lines have a lethal or sterile phenotype. The P{hsneo} construct carries a neomycin-resistance gene with a heat-shock promoter (allowing chemical selection in flies using G418), and bacterial sequences that allow plasmid rescue. First dataset of this type.
Insertion lines from this collection were assessed for inclusion in the BDGP Gene Disruption Project collection.
(Cooley et al., 1988, Spradling et al., 1999)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:639,303..639,303 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
61C7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
61C1-61C9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025579
(BDGP Project Members, 1994-1999)
hide Inserted Element
Construct P{hsneo}
Location-dependent
role
Size 5.075Kb
Associated alleles
Tn\neoRHsp70Bb.PS
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
l(3)neo1
(Cooley et al., 1988)
hide Alleles and Phenotypes
Causes alleles
l(3)neo11
(Cooley et al., 1988, BDGP Project Members, 1994-1999, Spradling et al., 1999, Matthews, 2006.1.25)
Lethality
References
lethal | recessive
(Cooley et al., 1988, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)neo1
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10240
P{hsneo}l(3)neo11 mwh1 red1 e1/TM3, Sb1 Ser1
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{hsneo}l(3)neo11
(Sinenko et al., 2004, Cooley et al., 1988, FlyBase, 1992-, )
Secondary FlyBase IDs
  • FBti0000069
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Sinenko et al., 2004, Dev. Biol. 273(1): 48--62
Yantar, a conserved arginine-rich protein is involved in Drosophila hemocyte development. [FBrf0179728]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Cooley et al., 1988, Science 239: 1121--1128
Insertional mutagenesis of the Drosophila genome with single P-elements. [FBrf0049003]
Personal communication to FlyBase
Matthews, 2006.1.25, neo1 insertion lines in FlyBase.
neo1 insertion lines in FlyBase. [FBrf0191508]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]