Dmel\P{lacW}cp309^s2172 Insertion

General Information
Symbol	Dmel\P{lacW}cp309^s2172	Species	D. melanogaster
Name		FlyBase ID	FBti0005647
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	cp309, Ecol\lacZ	Viability / fertility
Causes allele(s)	cp309^s2172, Ecol\lacZ^cp309-s2172	Stock availability	1 publicly available
LINE ID	l(3)s2172
Genomic Location
Chromosomal location	3L ( 71B3 )	Sequence location	3L:15,072,574..15,072,574 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:15,072,574..15,072,574 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	71B3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	71B4-71B5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034107 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	cp309 (BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	cp309^s2172 (BDGP Project Members, 1994-1999, Martinez-Campos et al., 2004, Spradling et al., 1999) Ecol\lacZ^cp309-s2172 (BDGP Project Members, 1994-1999)

Lethality References lethal \| embryonic stage \| maternal effect \| poor (Perrimon et al., 1996) lethal \| larval stage \| recessive (Perrimon et al., 1996) lethal \| recessive (Spradling et al., 1999, Martinez-Campos et al., 2004) viable (Martinez-Campos et al., 2004)
Sterility References
Phenotype Manifest In
	antennal sense organ & chordotonal organ (Martinez-Campos et al., 2004) centrosome & larval brain (Martinez-Campos et al., 2004) embryonic head & embryonic/first instar larval cuticle \| maternal effect (Perrimon et al., 1996) primary spermatocyte & centriole (Martinez-Campos et al., 2004) primary spermatocyte & spindle (Martinez-Campos et al., 2004)
Detailed Description
Statement Reference Homozygous cp309^s2172 flies are viable, but exhibit a severe `uncoordinated\' phenotype and die shortly after eclosion because they immediately get stuck in the food. The size and distribution of centrioles in wild-type and cp309^s2172 mutant interphase larval brain cells is indistinguishable. The same is true for testes cells. cp309^s2172 brain cells do not exhibit a higher mitotic index than control cells. Heterozygous cp309^s2172/+ flies have normal responses to a standard sound stimulus from electrodes placed in the antenna and head, while most (16/26) homozygous cp309^s2172 flies have no sound-evoked responses, and the amplitude of the response is also greatly reduced in those cp309^s2172 flies that do respond. In cp309^s2172/+ heterozygous antennae, the ciliary outer segments of the mechanosensory chordotonal neurons in the second antennal segment and of the chemosensory neurons in the third antennal segment are clearly visible. By contrast, in cp309^s2172 homozygous mutant antennae the sensory cilia in both the chordotonal organs and the olfactory bristles are absent or severely shortened and kinked, and the chordotonal cilia of the second segment fail to attach to the cuticle that connects the second and third antennal segments. In cp309^s2172 mutant testes, morphologically normal primary spermatocytes are formed that each contain two pairs of large, orthogonally arranged centrioles. However, as the spermatocytes mature, the centrioles often lose their orthogonal arrangement and partially fragment. cp309^s2172 mutant spermatocytes often form multipolar meiosis I spindles, with each spindle pole organised by at least one centriole. Although meiosis is highly abnormal in cp309^s2172 mutant spermatocytes, at least some cells develop into relatively normal looking sperm. However, the distribution of centrioles and nuclei in the cysts is often disorganised. Moreover, although the cp309^s2172 mutant sperm contain flagella, these are often nonmotile, even though electron microscopy reveals the sperm tails to be structurally normal. (Martinez-Campos et al., 2004) Germline clones produce eggs with patterning defects: heads are open. (Perrimon et al., 1996)
Expression Data
Reporter Expression

Additional Information
Statement Reference In wandering third instar larva, no detectable β-gal staining. (Meister and Braun, 1995.10)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)s2172 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	12089 w¹¹¹⁸; P{lacW}cp309^s2172/TM3, Sb¹
Linkouts
Comments
	Location 3L:15044733-15044734 confirmed by FlyBase alignment of dbGSS accession AQ034107 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P2089 (Meister and Braun, 1995.10) P{lacW}cp309^s2172 (FlyBase, 1992-) P{lacW}l(3)s2172^s2172 (FlyBase, 1992-, FlyBase, 2005, BDGP Project Members, 1994-1999) P{w^+mC=lacW}l(3)s2172^s2172 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001209
References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Martinez-Campos et al., 2004, J. Cell Biol. 165(5): 673--683 The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis. [FBrf0179327] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Perrimon et al., 1996, Genetics 144(4): 1681--1692 Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. [FBrf0091142] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}cp309s2172 Insertion

Dmel\P{lacW}cp309^s2172 Insertion