A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}Trls2325 Insertion

General Information
Symbol Dmel\P{lacW}Trls2325 Species D. melanogaster
Name FlyBase ID FBti0005653
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Trl Viability / fertility
Causes allele(s) Ecol\lacZTrl-s2325, Trls2325 Stock availability 2 publicly available
LINE ID l(3)s2325
Genomic Location
Chromosomal location 3L ( 70F4 ) Sequence location 3L:14,749,616..14,749,616 [+]
Map ( GBrowse ) detailed view FBti0051227 FBti0143125 FBti0013362_1 FBti0036149 FBti0068859 FBti0125690_2 FBti0125690_1 FBti0053159 FBti0059411 FBti0013362_3 FBti0013362_2 FBti0056510 FBti0055120 FBti0034368 FBti0011363 FBti0034081 FBti0047276 FBti0111207 FBti0028783 FBti0102836 FBti0029293 FBti0105808 FBti0109576 FBti0110508 FBti0005653 FBti0072259 FBti0029022 FBti0013245 FBti0112798 FBti0046987 FBti0048673 FBti0011702 FBti0012250_1 FBti0012250_2 FBti0030014 FBti0112674 FBti0039851 FBti0029657 FBti0028948 FBti0028253 FBti0053850 FBti0103065 FBti0066681 FBti0053933 FBti0035213 FBti0070473 FBti0107725 FBti0011370 FBti0069930 FBti0069581 FBti0021373 FBti0126677 FBti0068945 FBti0114853 FBti0103244 FBti0076088 FBti0066528 FBti0053746
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:14,749,616..14,749,616 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
70F4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
70F1-70F4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026386
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Trl
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZTrl-s2325
(BDGP Project Members, 1994-1999)
Trls2325
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Kehle et al., 1998, Katokhin et al., 2001, Dworkin and Gibson, 2006)
Lethality
References
lethal | larval stage | recessive
(Katokhin et al., 2001)
lethal | recessive
(Spradling et al., 1999, Dworkin and Gibson, 2006)
Sterility
References
hide Phenotype Manifest In
egg chamber
(Trunova et al., 2001)
mitosis & nuclear chromosome
(Trunova et al., 2001)
wing
(Dworkin and Gibson, 2006)
hide Detailed Description
Statement
Reference
Heterozygotes show a direct effect on the shape of the wing.
(Dworkin and Gibson, 2006)
Heterozygotes show the formation of "granular" chromatin during interphase and mitosis and high proportions of cells (which are arrested at the G2/M boundary) have hypercondensed chromatin. Cells with abnormal chromosome segregation are also seen. Heterozygous females have egg chambers with an abnormal number of trophocytes and defects in chromatin condensation.
(Trunova et al., 2001)
The formation of extra sex combs on the second and third legs in Pc4/+ flies is not enhanced by Trls2325.
(Kehle et al., 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
embryonic/larval salivary gland  
(Meister and Braun, 1995.10)
embryonic/larval midgut  
(Meister and Braun, 1995.10)
gastric caecum  
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)s2325
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12088
w1118; P{lacW}Trls2325/TM3, Sb1
Kyoto
111392
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Trls2325 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
Location 3L:14721775-14721776 confirmed by FlyBase alignment of dbGSS accession AQ026386 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2088
(Meister and Braun, 1995.10)
P{lacW}l(3)s2325s2325
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Trls2325
(FlyBase, 2005, FlyBase, 1992-)
P{w+mC=lacW}l(3)s2325s2325
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001213
hide References ( 12 )
Research paper
Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431
Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Katokhin et al., 2001, Genetika, Moscow 37(4): 467--474
[Molecular genetic analysis of Trithorax-like gene encoded transcriptional factor GAGA in Drosophila melanogaster]. [FBrf0136955]
Trunova et al., 2001, Genetika, Moscow 37(12): 1604--1615
[The effect of some mutations in the Trl gene on mitosis in embryonal and larval tissues and egg chamber morphology in Drosophila melanogaster] [FBrf0144909]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Kehle et al., 1998, Science 282(5395): 1897--1900
dMi-2, a hunchback-interacting protein that functions in polycomb repression. [FBrf0106056]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]