A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}RpS12s2783 Insertion

General Information
Symbol Dmel\P{lacW}RpS12s2783 Species D. melanogaster
Name FlyBase ID FBti0005663
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, RpS12 Viability / fertility
Causes allele(s) Ecol\lacZRpS12-s2783, RpS12s2783 Stock availability 2 publicly available
LINE ID l(3)s2783
Genomic Location
Chromosomal location 3L ( 69F5 ) Sequence location 3L:13,016,509..13,016,509 [+]
Map ( GBrowse ) detailed view FBti0115156 FBti0100380 FBti0042483 FBti0039640 FBti0041501 FBti0112201 FBti0067768 FBti0143590 FBti0099725 FBti0039483 FBti0011331 FBti0005663 FBti0045736 FBti0057604 FBti0065371 FBti0125672_1 FBti0125672_2 FBti0029993 FBti0055094 FBti0035040 FBti0028264 FBti0072544 FBti0039196 FBti0052492 FBti0049244
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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Chromosome (arm)
Sequence Location
3L:13,016,509..13,016,509 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
69F5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
69F5-69F6 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026389
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Netter et al., 1998)
RpS12
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZRpS12-s2783
(BDGP Project Members, 1994-1999, Netter et al., 1998)
RpS12s2783
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Netter et al., 1998)
Lethality
References
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
In wandering third instar larva, no detectable β-gal staining.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)s2783
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
12085
w1118; P{lacW}RpS12s2783/TM3, Sb1 Ser1
Kyoto
111390
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}RpS12s2783 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb1
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hide Comments
Location 3L:12997356-12997357 confirmed by FlyBase alignment of dbGSS accession AQ026389 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P2085
(Meister and Braun, 1995.10)
P{lacW}l(3)s2783s2783
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}RpS12s2783
(FlyBase, 2005, FlyBase, 1992-)
P{w+mC=lacW}l(3)s2783s2783
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0001217
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Netter et al., 1998, Genetics 149(1): 257--275
white+ transgene insertions presenting a dorsal/ventral pattern define a single cluster of homeobox genes that is silenced by the polycomb-group proteins in Drosophila melanogaster. [FBrf0102846]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase
Deal and Cook, 2002.1.4, Isolation and characterization of Df(3L)BSC10.
Isolation and characterization of Df(3L)BSC10. [FBrf0144465]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]