Dmel\P{lacW}drk^k02401 Insertion

General Information
Symbol	Dmel\P{lacW}drk^k02401	Species	D. melanogaster
Name		FlyBase ID	FBti0005877
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	drk, Ecol\lacZ	Viability / fertility
Causes allele(s)	drk^k02401, Ecol\lacZ^drk-k02401	Stock availability	2 publicly available
LINE ID	l(2)k02401
Genomic Location
Chromosomal location	2R ( 50A13 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	50A13 ( near gene of known cytology )
Cytological location (reported)	50A12-50A14 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	drk (BDGP Project Members, 1994-1999, Yan et al., 2009, Moressis et al., 2009) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	drk^k02401 (BDGP Project Members, 1994-1999, Botella et al., 2003, Roch et al., 1998, Spradling et al., 1999, Dworkin and Gibson, 2006, Yan et al., 2009, Moressis et al., 2009) Ecol\lacZ^drk-k02401 (BDGP Project Members, 1994-1999)

Lethality References lethal \| recessive (Roch et al., 1998, Spradling et al., 1999, Dworkin and Gibson, 2006)
Sterility References
Phenotype Manifest In
	wing \| posterior (Dworkin and Gibson, 2006)
Detailed Description
Statement Reference Olfactory association learning is lower in drk[k02401] heterozygotes compared to controls. (Moressis et al., 2009) A drk[k02401]/+ background does not suppress the ectopic cell divisions found in Uba1[B1]/Uba1[B2] trans-heterozygous eye discs. (Yan et al., 2009) Heterozygotes show a direct effect on the shape of the wing, having a significant effect in the posterior region, but no effect in the anterior region. (Dworkin and Gibson, 2006) Lethality occurs during third instar larval or pupal stages. Mutants do not show any visible disc abnormalities. (Roch et al., 1998)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k02401 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10372 y¹ w^67c23; P{lacW}drk^k02401/CyO
Kyoto	111070 w¹¹¹⁸; P{neoFRT}42D P{lacW}drk^k02401 /CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	drk^k02401 (Yan et al., 2009, Moressis et al., 2009) drk^P2 (Moressis et al., 2009) P{lacW}drk^k02401 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Moressis et al., 2009, J. Neurosci. 29(8): 2611--2625 A dual role for the adaptor protein DRK in Drosophila olfactory learning and memory. [FBrf0207513] Yan et al., 2009, J. Cell Sci. 122(9): 1461--1470 Impairment of ubiquitylation by mutation in Drosophila E1 promotes both cell-autonomous and non-cell-autonomous Ras-ERK activation in vivo. [FBrf0207806] Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431 Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Botella et al., 2003, Molec. Biol. Cell 14(1): 241--250 Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap. [FBrf0155942] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112 Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}drkk02401 Insertion

Dmel\P{lacW}drk^k02401 Insertion