Dmel\P{lacW}bur^k07130 Insertion

General Information
Symbol	Dmel\P{lacW}bur^k07130	Species	D. melanogaster
Name		FlyBase ID	FBti0006023
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	bur, Ecol\lacZ	Viability / fertility
Causes allele(s)	bur^k07130, Ecol\lacZ^bur-k07130	Stock availability	2 publicly available
LINE ID	l(2)k07130 l(2)07130
Genomic Location
Chromosomal location	2L ( 39A7-39B1 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	39A7-39B1 ( near gene of known cytology )
Cytological location (reported)	39B1-39B2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	bur (BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	bur^k07130 (BDGP Project Members, 1994-1999, Neufeld et al., 1998, Spradling et al., 1999, Long et al., 2006) Ecol\lacZ^bur-k07130 (BDGP Project Members, 1994-1999)

Lethality References lethal (van der Knaap et al., 2005) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	lamina (Long et al., 2006) medulla (Long et al., 2006) photoreceptor cell & axon (Long et al., 2006) photoreceptor cell & axon \| somatic clone \| cell autonomous (Long et al., 2006) photoreceptor cell R7 & growth cone (Long et al., 2006) photoreceptor cell R8 & growth cone (Long et al., 2006)
Detailed Description
Statement Reference Heterozygosity for bur[k07130] enhances the "held-out wing" phenotype of Usp7[Δ35] hemizygotes. (van der Knaap et al., 2010) R-cell axons migrate properly towards the posterior end of the eye disc and enter the optic stalk normally in homozygous larvae, and the axon tract in the optic stalk is morphologically indistinguishable from wild type. However, after exiting the optic stalk, the mutant R-cell axons fail to maintain their normal neighbour relationships, resulting in abnormally thick bundles in the lamina and medulla, crossing over of neighbouring axon bundles in the medulla and a less organised array of R7 and R8 growth cones in the medulla compared to wild type. The R7 and R8 growth cones are less expanded than normal and have abnormal morphology. Homozygous R-cell axons induced by the MARCM system in a heterozygous background do not expand upon reaching the target region in the optic lobe in 89% of cases and 16% have an abnormal topographic projection pattern. (Long et al., 2006) Flies transheterozygous for bur^k07130 and Pc¹ or Pc³ exhibit very strong wing-to-haltere transformations. In addition, males transheterozygous for bur^k07130 and either Pc¹ or Pc³ also show partial transformation of the anterior abdominal segments A4 towards A5 and carry sex combs on the second legs. (van der Knaap et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)07130 (Gene Disruption Project members, 2001-) l(2)k07130 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10174 y¹ w^67c23; P{lacW}bur^k07130/CyO
Kyoto	102531 y¹ w^67c23 ; P{lacW}bur^k07130 / CyO
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}bur^k07130 (FlyBase, 1992-) P{lacW}l(2)10523^k07130 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 10 )
Research paper	van der Knaap et al., 2010, Mol. Cell. Biol. 30(3): 736--744 Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. [FBrf0209736] Long et al., 2006, Genetics 172(3): 1633--1642 De novo GMP synthesis is required for axon guidance in Drosophila. [FBrf0190740] van der Knaap et al., 2005, Molec. Cell 17(5): 695--707 GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7. [FBrf0184151] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Neufeld et al., 1998, Genetics 148(1): 277--286 A genetic screen to identify components of the sina signaling pathway in Drosophila eye development. [FBrf0100609] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}burk07130 Insertion

Dmel\P{lacW}bur^k07130 Insertion