Dmel\P{lacW}sls^j1D7 Insertion

General Information
Symbol	Dmel\P{lacW}sls^j1D7	Species	D. melanogaster
Name		FlyBase ID	FBti0006132
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, sls	Viability / fertility
Causes allele(s)	Ecol\lacZ^sls-j1D7, sls^j1D7	Stock availability	2 publicly available
LINE ID	l(3)j1D7
Genomic Location
Chromosomal location	3L ( 62C2 )	Sequence location	3L:2,046,997..2,046,997 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:2,046,997..2,046,997 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	62C2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	62C1-62C2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AJ238577 (FlyBase, 1992-) AQ026295 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) sls (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^sls-j1D7 (BDGP Project Members, 1994-1999) sls^j1D7 (BDGP Project Members, 1994-1999, Kolmerer et al., 2000, Zhang et al., 2000, Machado and Andrew, 2000, Spradling et al., 1999)

Lethality References lethal \| embryonic stage \| recessive (Zhang et al., 2000) lethal \| recessive (Machado and Andrew, 2000, BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	mitosis & nuclear chromosome (Machado and Andrew, 2000) muscle fiber \| embryonic stage (Zhang et al., 2000)
Detailed Description
Statement Reference sls[j1D7] heterozygosity greatly increases the adult lethality of Df(3R)Mlp84B[P8]/Df(3R)dsx2M mutants. sls[j1D7] heterozygosity also exacerbates the pupal case elongation phenotype associated with Df(3R)Mlp84B[P8]/Df(3R)dsx2M or Mlp84B[P20]/Df(3R)dsx2M. sls[j1D7] heterozygosity reduces the viability of Mlp84B[P20]/Df(3R)dsx2M mutants to zero. sls[j1D7] heterozygosity in a Df(3R)Mlp84B[P8]/Df(3R)dsx2M mutant background leads to striking muscle abnormalities. Most muscle fibers are wavy and/or spindly in appearance. A significant number of fibers are torn or show some degree of fraying. Closer examination of the double mutants reveals a profound loss of sarcomeric structure of single muscle fibers from third instar fillet preparations. Neither Df(3R)Mlp84B[P8]/Df(3R)dsx2M hemizygotes, nor sls[j1D7] heterozygotes on their own display any obvious structural abnormalities at the light microscopic level. (Clark et al., 2007) 23.7% of nuclei in sls^e4/sls^j1D7 larval brain neuroblasts show chromosome undercondensation (under condensation of heterochromatin as well as euchromatin is seen). Additional mitotic defects such as premature sister chromatid separation, polyploidy, aneuploidy and chromosome breakage are also seen. (Machado and Andrew, 2000) Mutant embryos show grossly normal morphology, normal segmentation, normal epidermal denticle belts and normal patterning of the neuromusculature. They show greatly reduced movement compared to wild-type. Mature embryos show striking defects in individual muscle fibre morphology. The parallel myofilament array seen in wild-type muscles is disrupted and myofilaments are arranged irregularly, both longitudinally and transversely. The unit of a thick myosin filament surrounded by an array of thin actin filaments can form correctly, whereas the overall arrangement of myofilaments is largely disrupted. (Zhang et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j1D7 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10017 y¹ w^*; P{lacW}sls^j1D7/TM3, Sb¹
Kyoto	111002 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}sls^j1D7 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:2030529-2030530 confirmed by FlyBase alignment of dbGSS accession AQ026295 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}j1D7^j1D7 (FlyBase, 1992-) P{lacW}Ket^j1D7 (FlyBase, 1992-) P{lacW}l(3)j1D7^j1D7 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}sls^j1D7 (FlyBase, 2005, FlyBase, 1992-) P{lacW}Titin^j1D7 (FlyBase, 1992-) P{w^+mC=lacW}j1D7^j1D7 (FlyBase, 1992-) P{w^+mC=lacW}l(3)j1D7^j1D7 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001131 FBti0003047
References ( 11 )
Research paper	Clark et al., 2007, J. Cell Sci. 120(12): 2066--2077 The Drosophila muscle LIM protein, Mlp84B, cooperates with D-titin to maintain muscle structural integrity. [FBrf0200911] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Kolmerer et al., 2000, J. molec. Biol. 296(2): 435--448 Sequence and expression of the Kettin Gene in Drosophila melanogaster and Caenorhabditis elegans. [FBrf0125351] Machado and Andrew, 2000, J. Cell Biol. 151(3): 639--651 D-TITIN: a giant protein with dual roles in chromosomes and muscles. [FBrf0131354] Zhang et al., 2000, J. Cell Sci. 113(17): 3103--3115 Drosophila D-titin is required for myoblast fusion and skeletal muscle striation. [FBrf0130194] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}slsj1D7 Insertion

Dmel\P{lacW}sls^j1D7 Insertion