A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}vkgk00236 Insertion

General Information
Symbol Dmel\P{lacW}vkgk00236 Species D. melanogaster
Name FlyBase ID FBti0006279
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, vkg Viability / fertility
Causes allele(s) Ecol\lacZvkg-k00236, vkgk00236 Stock availability 2 publicly available
LINE ID l(2)k00236
Genomic Location
Chromosomal location 2L ( 25C1 ) Sequence location 2L:5,027,473..5,027,480 [-]
Map ( GBrowse ) GBrowse View Help
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:5,027,473..5,027,480 [-]
2L:5,027,471..5,027,471 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
25C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
25C1-25C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025683
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
vkg
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZvkg-k00236
(BDGP Project Members, 1994-1999)
vkgk00236
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Lethality
References
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
embryonic anterior Malpighian tubule
(Bunt et al., 2010)
hide Detailed Description
Statement
Reference
A small proportion of vkg[k00236] mutant embryos display anterior Malpighian tubule path-finding defects, and the anterior pair of tubules to not extend into the anterior half of the embryo.
(Bunt et al., 2010)
vkg[k00236] dominantly enhances the rough eye phenotype caused by expression of Zzzz\CTG[i480.Scer\UAS.cGa] under the control of Scer\GAL4[sev.PU].
(Garcia-Lopez et al., 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k00236
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10473
y1 w67c23; P{lacW}vkgk00236
Kyoto
111122
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}vkgk00236 P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:5027471-5027472 confirmed by FlyBase alignment of dbGSS accession AQ025683 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
8 bp insertion associated host repeat
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}vkgk00236
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bunt et al., 2010, Dev. Cell 19(2): 296--306
Hemocyte-Secreted Type IV Collagen Enhances BMP Signaling to Guide Renal Tubule Morphogenesis in Drosophila. [FBrf0211514]
Garcia-Lopez et al., 2008, PLoS ONE 3(2): e1595
Genetic and chemical modifiers of a CUG toxicity model in Drosophila. [FBrf0210239]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]