Dmel\P{lacW}RpI135^k16513 Insertion

General Information
Symbol	Dmel\P{lacW}RpI135^k16513	Species	D. melanogaster
Name		FlyBase ID	FBti0006354
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, RpI135	Viability / fertility
Causes allele(s)	Ecol\lacZ^{RpI135-k16513}, RpI135^k16513	Stock availability	2 publicly available
LINE ID	l(2)k16513
Genomic Location
Chromosomal location	2L ( 21C2 )	Sequence location	2L:404,303..404,310 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:404,303..404,310 [+] 2L:404,312..404,312 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	21C2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	21C1-21C2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034040 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) RpI135 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{RpI135-k16513} (BDGP Project Members, 1994-1999) RpI135^k16513 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Deal and Cook, 2005.3.10, Campbell and Tomlinson, 1998, Deal and Cook, 2001.4.24)

Lethality References lethal (Grewal et al., 2005) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999) viable (Campbell and Tomlinson, 1998)
Sterility References
Phenotype Manifest In
	embryonic/larval fat body \| somatic clone (Grewal et al., 2005)
Detailed Description
Statement Reference Homozygous mutant RpI135^k16513 clones can be rescued by genetically reducing the growth of surrounding cells by making them heterozygous for a dominant Minute RpS13¹ mutant. In this case, RpI135^k16513 mutant clones are recovered in all RpS13¹ heterozygous discs that are examined at 96hrs after clone induction, a time-point at which all clones are normally eliminated in a wild-type background. However, these clones colonise a significantly smaller area of RpS13¹/+ discs compared with wild-type clones (28.8% compared to 67.8% of the total disc area), indicating that they are still growth impaired. Compared to surrounding heterozygote RpS13¹ or wild-type cells, RpI135^k16513 mutant clone cells exhibit no significant change in either cell size or cell-cycle phasing. (Grewal et al., 2005) Homozygous mutant RpI135^k16513 clone survival can be rescued by co-expression of the apoptosis inhibitor, BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^αTub84B.PL. However, the clones are still growth defective, indicating that the reduced growth and viability of mutant clones is not simply due to increased cell death. (Grewal et al., 2005) Homozygous RpI135^k16513 mutant larvae hatch and are viable. However, they remain as growth-arrested L1 larvae and survive for up to 8 days. RpI135^k16513 homozygous cells in somatic clones in the larval fat body are small and have decreased DNA content compared to surrounding endoreplicated cells. RpI135^k16513 homozygous somatic clones in the wing disc are about 50% smaller than wild-type twin-spots, but the cells in these clones are of normal size, and show no shift in cell-cycle phasing compared to wild-type siblings. The viability of these clones decreases with time after induction. (Grewal et al., 2005) Viable in combination with RpI135^k16513. (Campbell and Tomlinson, 1998)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k16513 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11186 y¹ w^67c23; P{lacW}RpI135^k16513/CyO
Kyoto	111359 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}RpI135^k16513 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:404312-404313 confirmed by FlyBase alignment of dbGSS accession AQ034040 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005) 8 bp insertion associated host repeat
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k16513^k16513 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}RpI135^k16513 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Grewal et al., 2005, Nature Cell Biol. 7(3): 295--302 Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development. [FBrf0184182] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Campbell and Tomlinson, 1998, Development 125(22): 4483--4493 The roles of the homeobox genes aristaless and Distal-less in patterning the legs and wings of Drosophila. [FBrf0105784] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Deal and Cook, 2005.3.10, Isolation and characterization of Df(2L)BSC106. Isolation and characterization of Df(2L)BSC106. [FBrf0182721] Deal and Cook, 2001.4.24, Isolation and Characterization of Df(2L)BSC4. Isolation and Characterization of Df(2L)BSC4. [FBrf0136028]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}RpI135k16513 Insertion

Dmel\P{lacW}RpI135^k16513 Insertion