Dmel\P{lacW}Vps28^k16503 Insertion

General Information
Symbol	Dmel\P{lacW}Vps28^k16503	Species	D. melanogaster
Name		FlyBase ID	FBti0006358
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Vps28	Viability / fertility
Causes allele(s)	Ecol\lacZ^Vps28-k16503, Vps28^k16503	Stock availability	2 publicly available
LINE ID	l(2)k16503
Genomic Location
Chromosomal location	2R ( 44A4 )	Sequence location	2R:3,967,507..3,967,507 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:3,967,507..3,967,507 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	44A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	44A4-44A5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026107 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Vps28 (BDGP Project Members, 1994-1999, Ahmad et al., 2009, Sevrioukov et al., 2005, Geng and MacDonald, 2007, Saja et al., 2010)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Vps28-k16503 (BDGP Project Members, 1994-1999) Vps28^k16503 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Ahmad et al., 2009, Sevrioukov et al., 2005, Geng and MacDonald, 2007, Saja et al., 2010)

Lethality References lethal \| embryonic stage \| germline clone (Sevrioukov et al., 2005) lethal \| first instar larval stage (Sevrioukov et al., 2005) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References male sterile (Sevrioukov et al., 2005)
Phenotype Manifest In
	eye photoreceptor cell \| somatic clone (Sevrioukov et al., 2005) fat body \| conditional qualifier \| somatic clone (Juhász et al., 2008) ommatidium \| somatic clone (Sevrioukov et al., 2005) pole cell \| germline clone (Sevrioukov et al., 2005) spermatid (Sevrioukov et al., 2005) spermatozoon (Sevrioukov et al., 2005)
Detailed Description
Statement Reference A Vps28[k16503] background does not enhance or suppress the eye degeneration phenotype found upon expression of Ppt1[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF]. (Saja et al., 2010) A single copy of Vps28[k16503] does not cause an eye phenotype. (Ahmad et al., 2009) A single copy of Vps28[k16503] significantly enhances the Hsap\CHMP2B[Intron5.Scer\UAS] Scer\GAL4[GMR.PF] phenotype in 1-day-old flies. (Ahmad et al., 2009) Vps28[k16503] and Pi3K59F[Δm22] double mutant clones generated in the fat-body show accumulation of autophagosomes under fed conditions, compared to none observed in Pi3K59F[Δm22] single mutant clones. (Juhász et al., 2008) Fat-body cells mutant for Vps28[k16503] show an accumulation of autophagosomes in fed conditions. These cells show a reduction in autolysosomes in response to 4-hour starvation conditions. (Juhász et al., 2008) Flies with eyes carrying Vps28^k16503 clones exhibit a disorganization of the ommatidia that is externally visible as roughness of the compound eye. The majority of ommatidia within these eyes contain no visible defects, while some ommatidia are missing photoreceptor cells, some contain extra cells and others contain a full complement of cells but are still not oriented correctly. Cells within the retina contain abnormally large multivesicular bodies that have internal vesicles. Embryos produced from homozygous Vps28^k16503 germ cells exhibit major defects early in development. About one-half of these eggs are not fertilized although the micropyles are not malformed. Embryos that are fertilized and undergo nuclear divisions show developmental arrest before complete cellularization. These embryos frequently show irregular distribution of nuclei and have no pole cells. Cells that do form display an aberrant droplet-like shape. The embryos exhibit defects in the actin cytoskeleton, with some regions being devoid of actin and others containing enlarged actin bundles. Vps28^k16503 males that express Vps28^Scer\UAS.cSa under the control of Scer\GAL4^arm.PS are infertile and possess very few motile sperm. These males show a defect at sperm individualization, where the appearance of actin cones is disorganised compared to wild-type males. (Sevrioukov et al., 2005) Eyes that carry Vps28^k16503, or^49h double mutant clones show the same two phenotypes as Vps28^k16503 and or^49h single mutant clones. (Sevrioukov et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k16503 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11182 y¹ w^67c23; P{lacW}Vps28^k16503/CyO
Kyoto	103010 y¹ w^67c23 ; P{lacW}Vps28^k16503 / CyO
Linkouts
Comments
	Location 2R:3591815-3591816 confirmed by FlyBase alignment of dbGSS accession AQ026107 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	k16503 (Geng and MacDonald, 2007) l(2)k16503 (Sevrioukov et al., 2005) P{lacW}l(2)k16503^k16503 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}Vps28^k16503 (FlyBase, 2005, FlyBase, 1992-, Saja et al., 2010) vps28^l(2)K16503 (Ahmad et al., 2009)
Secondary FlyBase IDs

References ( 12 )
Research paper	Saja et al., 2010, Neurobiol. Disease 40(1): 135--145 Identifying cellular pathways modulated by Drosophila palmitoyl-protein thioesterase 1 function. [FBrf0211567] Ahmad et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(29): 12168--12173 Genetic screen identifies serpin5 as a regulator of the toll pathway and CHMP2B toxicity associated with frontotemporal dementia. [FBrf0208495] Juhász et al., 2008, J. Cell Biol. 181(4): 655--666 The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila. [FBrf0204844] Geng and MacDonald, 2007, Fly 1(5): 259--267 Identification of genes that influence gurken expression. [FBrf0205043] Sevrioukov et al., 2005, Molec. Biol. Cell 16(5): 2301--2312 A mutation in dVps28 reveals a link between a subunit of the endosomal sorting complex required for transport-I complex and the actin cytoskeleton in Drosophila. [FBrf0188067] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Vps28k16503 Insertion

Dmel\P{lacW}Vps28^k16503 Insertion