Dmel\P{lacW}ebi^k16213 Insertion

General Information
Symbol	Dmel\P{lacW}ebi^k16213	Species	D. melanogaster
Name		FlyBase ID	FBti0006364
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	ebi, Ecol\lacZ	Viability / fertility
Causes allele(s)	ebi^k16213, Ecol\lacZ^ebi-k16213	Stock availability	3 publicly available
LINE ID	l(2)k16213
Genomic Location
Chromosomal location	2L ( 21C2 )	Sequence location	2L:415,191..415,191 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Controlled Vocabulary Terms wing semi-lethal Genes ebi References Marygold et al., 2011, Fly 5(4): 291--303
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:415,191..415,191 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	21C2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	21C2-21C3 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026103 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	ebi (BDGP Project Members, 1994-1999, Marygold et al., 2011) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	ebi^k16213 (BDGP Project Members, 1994-1999, Tsuda et al., 2002, Dong et al., 1999, Boulton et al., 2000, Spradling et al., 1999, Marygold et al., 2011) Ecol\lacZ^ebi-k16213 (BDGP Project Members, 1994-1999)

Lethality References lethal (Marygold et al., 2011) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Boulton et al., 2000, Marygold et al., 2011) semi-lethal (Marygold et al., 2011)
Sterility References
Phenotype Manifest In
	crystal cell (Milchanowski et al., 2004) wing (Marygold et al., 2011)
Detailed Description
Statement Reference ebi[+t7.5] rescues the homozygous lethality of ebi[k16213]. (Marygold et al., 2011) ebi[P7]/ebi[k16213] wings are 82% the size of control wings. Gross defects in wing patterning are not apparent. There is no significant decrease in the size of other organs or the whole organism. (Marygold et al., 2011) Heterozygosity for ebi[k16213] enhances the small wing phenotype induced by Scer\GAL4[Bx-MS1096]-mediated expression of Pi3K92E[D954A.Scer\UAS.T:Hsap\MYC]. Heterozygosity for ebi[k16213] enhances the notched wing margin phenotype seen in sd[ETX4] hemizygous males. (Marygold et al., 2011) ebi^k16213/+ ; sinah¹/sinah¹ flies have an extra dorsocentral bristle; on average flies contain 0.82 extra bristles and one or more extra bristles are seen in 36% of males and 68% of females. The dorsocentral bristles are aligned and spaced in an anterior to posterior row as in wild type, but because of the extra bristle(s), the row extends further anteriorly than in wild type. (Cooper, 2007) The average number of crystal cells per embryo is reduced (to 13.6) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). Mutant stage 14 embryos show cell cycle defects in the head region. (Milchanowski et al., 2004) Enhances the rough eye phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH, increasing the irregular arrangement of the ommatidial facets. A large number of inter-ommatidial bristle duplications are also seen. (Boulton et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k16213 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	11166 y¹ w^67c23; P{lacW}ebi^k16213/CyO
Kyoto	108864 y¹ w^67c23; P{lacW}ebi^k16213/CyO 111356 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}ebi^k16213 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:415191-415192 confirmed by FlyBase alignment of dbGSS accession AQ026103 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}ebi^k16213 (FlyBase, 2005, FlyBase, 1992-, Marygold et al., 2011) P{lacW}l(2)k16213^k16213 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Marygold et al., 2011, Fly 5(4): 291--303 Genetic characterization of ebi reveals its critical role in Drosophila wing growth. [FBrf0216882] Cooper, 2007, Mech. Dev. 124(7-8): 584--591 In vivo function of a novel Siah protein in Drosophila. [FBrf0200839] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Milchanowski et al., 2004, Genetics 168(1): 325--339 Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297] Tsuda et al., 2002, Cell 110(5): 625--637 An EGFR/Ebi/Sno pathway promotes delta expression by inactivating Su(H)/SMRTER repression during inductive Notch signaling. [FBrf0151813] Boulton et al., 2000, EMBO J. 19(20): 5376--5386 A role for ebi in neuronal cell cycle control. [FBrf0131258] Dong et al., 1999, Genes Dev. 13(8): 954--965 ebi regulates epidermal growth factor receptor signaling pathways in Drosophila. [FBrf0108171] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}ebik16213 Insertion

Dmel\P{lacW}ebi^k16213 Insertion