A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Egmk14708 Insertion

General Information
Symbol Dmel\P{lacW}Egmk14708 Species D. melanogaster
Name FlyBase ID FBti0006401
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Egm Viability / fertility
Causes allele(s) Ecol\lacZEgm-k14708, Egmk14708 Stock availability 2 publicly available
LINE ID l(2)k14708
Genomic Location
Chromosomal location 2R ( 48A3 ) Sequence location 2R:7,506,467..7,506,467 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0049631 FBti0053063 FBti0109036 FBti0036986 FBti0104913 FBti0036873 FBti0006442 FBti0108356 FBti0023443 FBti0036928 FBti0055969 FBti0125108_1 FBti0125108_2 FBti0100856 FBti0007646 FBti0010862 FBti0048773 FBti0006401 FBti0025793 FBti0046457 FBti0110251 FBti0018132
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:7,506,467..7,506,467 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
48A3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
48A3-48A5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034037
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Egm
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZEgm-k14708
(BDGP Project Members, 1994-1999)
Egmk14708
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Mourikis, 2006.3.2, Roch et al., 1998)
Lethality
References
lethal | larval stage
(Mourikis, 2006.3.2)
lethal | recessive
(Roch et al., 1998, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
imaginal disc
(Roch et al., 1998)
hide Detailed Description
Statement
Reference
Expression of the Egm+tMa transgene rescues the lethality of Egmk14708 flies.
(Mourikis et al., 2006)
Egmk14708 heterozygous females live longer (18.4% more on average) than control flies. A similar longevity phenotype is observed for Egmk14708 flies kept at a higher temperature (29oC).
(Mourikis et al., 2006)
Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are of normal size and shape.
(Roch et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k14708
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11133
y1 w67c23; P{lacW}Egmk14708/CyO
Kyoto
111342
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Egmk14708 /CyO y+
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hide Comments
Location 2R:7134869-7134870 confirmed by FlyBase alignment of dbGSS accession AQ034037 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}Egmk14708
(FlyBase, 1992-, )
P{lacW}l(2)k14708k14708
(FlyBase, 1992-, FlyBase, 2005, BDGP Project Members, 1994-1999)
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Mourikis et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(5): 1307--1312
Enigma, a mitochondrial protein affecting lifespan and oxidative stress response in Drosophila. [FBrf0191453]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Daborn et al., 2001, Mol. Genet. Genomics 266(4): 556--563
DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid. [FBrf0145128]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Roch et al., 1998, Mol. Gen. Genet. 257(2): 103--112
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Mourikis, 2006.3.2, Enigma.
Enigma. [FBrf0191663]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]