A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Xbp1k13803 Insertion

General Information
Symbol Dmel\P{lacW}Xbp1k13803 Species D. melanogaster
Name FlyBase ID FBti0006425
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Xbp1 Viability / fertility
Causes allele(s) Ecol\lacZXbp1-k13803, Xbp1k13803 Stock availability 2 publicly available
LINE ID l(2)k13803
Genomic Location
Chromosomal location 2R ( 57C3 ) Sequence location 2R:17,031,201..17,031,201 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0019044 FBti0111122 FBti0075062 FBti0116086 FBti0026005 FBti0010859 FBti0113462 FBti0070018 FBti0074704 FBti0113315 FBti0100134 FBti0006425 FBti0044840 FBti0044844 FBti0021925
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:17,031,201..17,031,201 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
57C3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
57C1-57C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034031
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Xbp1
(BDGP Project Members, 1994-1999, Souid et al., 2007, Loewen and Feany, 2010)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZXbp1-k13803
(BDGP Project Members, 1994-1999)
Xbp1k13803
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Roch et al., 1998, Souid et al., 2007, Loewen and Feany, 2010)
Lethality
References
lethal
(Souid et al., 2007)
lethal | first instar larval stage | recessive
(Souid et al., 2007)
lethal | recessive
(Spradling et al., 1999, Roch et al., 1998)
lethal | recessive | second instar larval stage
(Ryoo et al., 2007)
Sterility
References
hide Phenotype Manifest In
autophagic vacuole & larval fat body | somatic clone | cell autonomous
(Arsham and Neufeld, 2009)
imaginal disc
(Ryoo et al., 2007)
lysosome & larval fat body | somatic clone | cell autonomous
(Arsham and Neufeld, 2009)
hide Detailed Description
Statement
Reference
A heterozygous Xbp1[k13803] background significantly increases the level of apoptosis mediated by expression of Hsap\MAPT[R406W.Scer\UAS] under the control of Scer\GAL4[elav-C155].
(Loewen and Feany, 2010)
Heterozygous Xbp1[k13803] mutants do not exhibit an increase in apoptosis.
(Loewen and Feany, 2010)
Xbp1[k13803] clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.
(Arsham and Neufeld, 2009)
At day 4 of development, Xbp1[k13803] larvae are significantly retarded in their growth compared to their heterozygous siblings. Xbp1[k13803]/Df(2R)F36 mutant larvae lack imaginal discs.
(Ryoo et al., 2007)
A Xbp1[k13803]/+ background significantly accelerates the retinal degeneration of ninaE[G69D]/+ flies: the pseudopupil of approximately 30% of Xbp1[k13803]/+ ninaE[G69D]/+ flies starts to disappear by day 8 (i.e. 8 days earlier than in ninaE[G69D]/+ flies) and no pseudopupils are observed after day 16.
(Ryoo et al., 2007)
95% of homozygotes hatch (these animals appear indistinguishable from controls at the time of hatching). The homozygous larvae remain at the first instar stage, become sluggish and sick before dying after two to four says.
(Souid et al., 2007)
Lethality occurs at an early stage of development, mutants are underdeveloped even under prolonged incubation periods.
(Roch et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k13803
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11104
y1 w67c23; P{lacW}Xbp1k13803/CyO
Kyoto
111331
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Xbp1k13803 /CyO y+
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hide Comments
Location 2R:16651278-16651279 confirmed by FlyBase alignment of dbGSS accession AQ034031 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k13803k13803
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Xbp1k13803
(FlyBase, 2005, FlyBase, 1992-, )
Xbp1k13803
(Loewen and Feany, 2010)
Secondary FlyBase IDs
hide References ( 13 )
Research paper
Loewen and Feany, 2010, PLoS ONE 5(9): e13084
The unfolded protein response protects from tau neurotoxicity in vivo. [FBrf0211994]
Arsham and Neufeld, 2009, PLoS ONE 4(6): e6068
A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway. [FBrf0208155]
Ryoo et al., 2007, EMBO J. 26(1): 242--252
Unfolded protein response in a Drosophila model for retinal degeneration. [FBrf0194899]
Souid et al., 2007, Dev. Genes Evol. 217(2): 159--167
The xbp-1 gene is essential for development in Drosophila. [FBrf0193446]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Roch et al., 1998, Mol. Gen. Genet. 257(2): 103--112
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]