A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}Nlek13714 Insertion

General Information
Symbol Dmel\P{lacW}Nlek13714 Species D. melanogaster
Name FlyBase ID FBti0006427
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) CG2807, Ecol\lacZ, Nle Viability / fertility
Causes allele(s) CG2807k13714, Ecol\lacZNle-k13714, Nlek13714 Stock availability 2 publicly available
LINE ID l(2)k13714
Genomic Location
Chromosomal location 2L ( 21E2 ) Sequence location 2L:568,373..568,380 [-]
Map ( GBrowse )
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:568,373..568,380 [-]
2L:568,371..568,371 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
21E2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
21C7-21C8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026066
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG2807
(FlyBase, 1996-)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Nle
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
CG2807k13714
(FlyBase, 1996-)
Ecol\lacZNle-k13714
(BDGP Project Members, 1994-1999)
Nlek13714
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Deal and Cook, 2005.3.10, FlyBase, 1996-)
Lethality
References
lethal | recessive
(Spradling et al., 1999)
viable
(Royet et al., 1998)
Sterility
References
male sterile
(Royet et al., 1998)
hide Phenotype Manifest In
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Statement
Reference
Dominantly suppresses wing margin and vein phenotypes of Nnd-1 and Nnd-2 though wing veins are interrupted due to overactivation of N signalling pathway. Dominantly suppresses wing margin and vein phenotypes of Nnd-3, and dx1. Enhances the bristle and wing vein phenotypes of gain of function NAx-1. Reveals sub-threshold effect of dxhs.PB, in effect on wing veins.
(Royet et al., 1998)
Homozygotes are viable, morphologically normal and male sterile.
(Royet et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k13714
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11099
y1 w67c23; P{lacW}Nlek13714 CG2807k13714/CyO
Kyoto
111330
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Nlek13714 P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:568371-568372 confirmed by FlyBase alignment of dbGSS accession AQ026066 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion associated host repeat; the sequence is from GB:AQ026066; reported to be 310 bp 5' to the start of the open reading frame in FBrf0105918
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k13714k13714
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Nlek13714
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Royet et al., 1998, EMBO J. 17(24): 7351--7360
Notchless encodes a novel WD40-repeat-containing protein that modulates notch signaling activity. [FBrf0105918]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Deal and Cook, 2005.3.10, Isolation and characterization of Df(2L)BSC107.
Isolation and characterization of Df(2L)BSC107. [FBrf0182722]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]