Dmel\P{lacW}nrv2^k13315 Insertion

General Information
Symbol	Dmel\P{lacW}nrv2^k13315	Species	D. melanogaster
Name		FlyBase ID	FBti0006443
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, nrv2	Viability / fertility
Causes allele(s)	Ecol\lacZ^nrv2-k13315, nrv2^k13315	Stock availability	2 publicly available
LINE ID	l(2)k13315
Genomic Location
Chromosomal location	2L ( 27B1 )	Sequence location	2L:6,792,138..6,792,138 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:6,792,138..6,792,138 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	27B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	27B1-27B2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026055 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) nrv2 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^nrv2-k13315 (BDGP Project Members, 1994-1999) nrv2^k13315 (BDGP Project Members, 1994-1999, Kaufmann et al., 2002, Spradling et al., 1999, Paul et al., 2003)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous clones in the third larval instar tracheal system are only occasionally recovered after they have been induced at the late blastoderm/early gastrulation stage. (Baer et al., 2007) When expressed under the control of Scer\GAL4^e22c, nrv1^{Scer\UAS.T:Zzzz\FLAG} does not rescue the dye diffusion phenotype across the salivary gland epithelium observed in nrv2^k13315 mutants. (Genova and Fehon, 2003) In nrv2^k13315 homozygous mutants the adherens junction remains intact, with the regular spacing of plasma membranes maintained. However, the septae normally located between these membranes are reduced in number or absent. The paracellular diffusion barrier is disrupted in salivary glands of nrv2^k13315 homozygous embryos. nrv2^k13315/nrv2^k13315 somatic clones generated in larvae (approx. 72 hours after egg laying) do not survive in adult tissues. (Genova and Fehon, 2003) When expressed under the control of Scer\GAL4^e22c, nrv2^{1.Scer\UAS.T:Zzzz\FLAG} rescues the dye diffusion phenotype across the salivary gland epithelium observed in nrv2^k13315 mutants. (Genova and Fehon, 2003) nrv2^k13315/nrv2^k04223 tracheal phenotypes are not suppressed by nrv1^Scer\UAS.cPa; Scer\GAL4^e22c. nrv2^k13315/nrv2^23B tracheal phenotypes are not suppressed by nrv3^Scer\UAS.cPa; Scer\GAL4^e22c. (Paul et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k13315 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11086 y¹ w^67c23; P{lacW}nrv2^k13315/CyO
Kyoto	114351 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}nrv2^k13315 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:6792138-6792139 confirmed by FlyBase alignment of dbGSS accession AQ026055 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k13315^k13315 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}nrv2^k13315 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 14 )
Research paper	Baer et al., 2007, Genetics 176(4): 2279--2291 A clonal genetic screen for mutants causing defects in larval tracheal morphogenesis in Drosophila. [FBrf0201252] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Genova and Fehon, 2003, J. Cell Biol. 161(5): 979--989 Neuroglian, Gliotactin, and the Na+/K+ ATPase are essential for septate junction function in Drosophila. [FBrf0160546] Paul et al., 2003, Development 130(20): 4963--4974 The Na+/K+ ATPase is required for septate junction function and epithelial tube-size control in the Drosophila tracheal system. [FBrf0162083] Kaufmann et al., 2002, Neuron 34(1): 27--38 Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis. [FBrf0147174] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Deal and Cook, 2001.12.20, Isolation and characterization of Df(2L)BSC9. Isolation and characterization of Df(2L)BSC9. [FBrf0141852] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}nrv2k13315 Insertion

Dmel\P{lacW}nrv2^k13315 Insertion