Dmel\P{lacW}Khc^k13314 Insertion

General Information
Symbol	Dmel\P{lacW}Khc^k13314	Species	D. melanogaster
Name		FlyBase ID	FBti0006444
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Khc	Viability / fertility
Causes allele(s)	Ecol\lacZ^Khc-k13314, Khc^k13314	Stock availability	2 publicly available
LINE ID	l(2)k13314
Genomic Location
Chromosomal location	2R ( 53A4 )	Sequence location	2R:12,159,268..12,159,268 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:12,159,268..12,159,268 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	53A4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	53A3-53A5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026054 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Khc (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Khc-k13314 (BDGP Project Members, 1994-1999) Khc^k13314 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Whited et al., 2004)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous mutants have swelling in the dendrites. (Medina et al., 2008) Khc[k13314] capt[K304] double heterozygotes have swelling in the dendrites (this phenotype is not seen in either single heterozygote). capt[K304] Khc[k13314] double homozygotes show a higher frequency of Act5C[Scer\UAS.T:Avic\GFP] puncta in the dendrites and dendritic swellings than seen in either the capt[K304] or Khc[k13314] single homozygotes respectively. In addition, the double homozygotes show formation of elongate actin rods (expressing Act5C[Scer\UAS.T:Avic\GFP]), a phenotype that is not seen in either single homozygote. (Medina et al., 2008) The Gl¹/+ adult eye phenotype and the loss of photoreceptor nuclei from the retina in late third instar Gl¹/+ larvae are partially suppressed by Khc^k13314/+. The loss of photoreceptor nuclei from the retina in late third instar Gl^Δ.Scer\UAS; Scer\GAL4^Glass38-1 larvae is significantly suppressed by Khc^k13314/+. Mislocalisation of the Bolwig\'s organ nuclei in Gl^Δ.Scer\UAS/Scer\GAL4^elav.PLu second instar larvae is partially suppressed by Khc^k13314/+ . (Whited et al., 2004) S^48-5/Khc^k13314 mutants show 2.87%+-2.04 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k13314 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11084 y¹ w^67c23; P{lacW}Khc^k13314/CyO
Kyoto	111322 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Khc^k13314 /CyO y⁺
Linkouts
Comments
	Location 2R:11786625-11786626 determined by FlyBase alignment of dbGSS accession AQ026054 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Khc^k13314 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(2)k13314^k13314 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 10 )
Research paper	Medina et al., 2008, PLoS ONE 3(8): e3054 The actin-binding protein capulet genetically interacts with the microtubule motor kinesin to maintain neuronal dendrite homeostasis. [FBrf0205725] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Whited et al., 2004, Development 131(19): 4677--4686 Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons. [FBrf0180149] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Khck13314 Insertion

Dmel\P{lacW}Khc^k13314 Insertion