A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}Khck13219 Insertion

General Information
Symbol Dmel\P{lacW}Khck13219 Species D. melanogaster
Name FlyBase ID FBti0006447
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Khc Viability / fertility
Causes allele(s) Ecol\lacZKhc-k13219, Khck13219 Stock availability 2 publicly available
LINE ID l(2)k13219
Genomic Location
Chromosomal location 2R ( 53A4 ) Sequence location 2R:12,159,268..12,159,268 [-]
Map ( GBrowse ) detailed view FBti0046239 FBti0025049 FBti0040157 FBti0052409 FBti0046663 FBti0006444 FBti0006447 FBti0033617 FBti0026224 FBti0112301 FBti0040246 FBti0041568 FBti0125214_2 FBti0125214_1 FBti0048986 FBti0024950 FBti0113006
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:12,159,268..12,159,268 [-]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
53A4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
53B1-53B2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026051
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Khc
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZKhc-k13219
(BDGP Project Members, 1994-1999)
Khck13219
(BDGP Project Members, 1994-1999)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
The Gl1/+ adult eye phenotype and the loss of photoreceptor nuclei from the retina in late third instar Gl1/+ larvae are partially suppressed by Khck13219/+.
(Whited et al., 2004)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k13219
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11077
y1 w67c23; P{lacW}Khck13219/CyO
Kyoto
111321
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Khck13219 /CyO y+
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hide Comments
Location 2R:11786625-11786626 determined by FlyBase alignment of dbGSS accession AQ026051 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}Khck13219
(FlyBase, 1992-)
P{lacW}l(2)k13219k13219
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Whited et al., 2004, Development 131(19): 4677--4686
Dynactin is required to maintain nuclear position within postmitotic Drosophila photoreceptor neurons. [FBrf0180149]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]