Dmel\P{lacW}ex^k12913 Insertion

General Information
Symbol	Dmel\P{lacW}ex^k12913	Species	D. melanogaster
Name		FlyBase ID	FBti0006456
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, ex	Viability / fertility
Causes allele(s)	Ecol\lacZ^ex-k12913, ex^k12913	Stock availability	3 publicly available
LINE ID	l(2)k12913
Genomic Location
Chromosomal location	2L ( 21C2 )	Sequence location	2L:431,832..431,832 [-]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:431,832..431,832 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	21C2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	21C3-21C4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026045 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) ex (BDGP Project Members, 1994-1999, Lee et al., 2008)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^ex-k12913 (BDGP Project Members, 1994-1999) ex^k12913 (Lee et al., 2008, BDGP Project Members, 1994-1999)

Lethality References viable (Lee et al., 2008)
Sterility References
Phenotype Manifest In
	spermatogonial cyst \| cell non-autonomous \| somatic clone (Sun et al., 2008)
Detailed Description
Statement Reference ex[k12913] homozygous mutant clones generated in the male germline develop normally. 16 cells are observed per cyst, and cell size and morphology are indistinguishable from neighbouring control cells. However, non-autonomous germline over-proliferation is observed in non-mutant spermatogonial cysts. (Sun et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k12913 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	11067 y¹ w^67c23; P{lacW}ex^k12913/CyO
Kyoto	108862 y¹ w^67c23; P{lacW}ex^k12913/CyO 111316 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}ex^k12913 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:431831-431832 determined by FlyBase alignment of dbGSS accession AQ026045 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}ex^k12193 (Parks et al., 2004) P{lacW}ex^k12913 (FlyBase, 2005, FlyBase, 1992-, Lee et al., 2008) P{lacW}l(2)k12913^k12913 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Sun et al., 2008, Dev. Dyn. 237(1): 270--275 Genes of Hippo signaling network act unconventionally in the control of germline proliferation in Drosophila. [FBrf0200427] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Lee et al., 2008, Curr. Biol. 18(24): Supplemental Data. [FBrf0207609] Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Deal and Cook, 2002.7.14, Isolation and characterization of Df(2L)BSC16. Isolation and characterization of Df(2L)BSC16. [FBrf0150855]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}exk12913 Insertion

Dmel\P{lacW}ex^k12913 Insertion