A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}RFeSPk11704 Insertion

General Information
Symbol Dmel\P{lacW}RFeSPk11704 Species D. melanogaster
Name FlyBase ID FBti0006467
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, RFeSP Viability / fertility
Causes allele(s) Ecol\lacZRFeSP-k11704, RFeSPk11704 Stock availability 2 publicly available
LINE ID l(2)k11704
Genomic Location
Chromosomal location 2L ( 22A3 ) Sequence location 2L:1,614,120..1,614,120 [+]
Map ( GBrowse ) detailed view FBti0019105 FBti0040838 FBti0124501 FBti0025860 FBti0006467
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:1,614,120..1,614,120 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
22A3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
22A3--22A4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
22A3-22A4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034029
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(Gelbart, 1999.7.20, BDGP Project Members, 1994-1999)
RFeSP
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZRFeSP-k11704
(Gelbart, 1999.7.20, BDGP Project Members, 1994-1999)
RFeSPk11704
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Gelbart, 1999.7.20)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
lysosome & larval fat body | somatic clone | cell autonomous
(Arsham and Neufeld, 2009)
hide Detailed Description
Statement
Reference
RFeSP[k11704] clones in fat bodies from well-fed early third instar larvae accumulate lysosomes in an unusually perinuclear concentration, as indicated by LysoTracker staining.
(Arsham and Neufeld, 2009)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k11704
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
11048
y1 w67c23; P{lacW}RFeSPk11704/CyO
Kyoto
111311
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}RFeSPk11704 P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:1614120-1614121 confirmed by FlyBase alignment of dbGSS accession AQ034029 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k11704
(Gelbart, 1999.7.20, FlyBase, 1992-)
P{lacW}l(2)k11704k11704
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}RFeSPk11704
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0013083
hide References ( 9 )
Research paper
Arsham and Neufeld, 2009, PLoS ONE 4(6): e6068
A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway. [FBrf0208155]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gelbart, 1999.7.20, BDGP lines.
BDGP lines. [FBrf0109761]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]