Dmel\P{lacW}Ark^k11502 Insertion

General Information
Symbol	Dmel\P{lacW}Ark^k11502	Species	D. melanogaster
Name		FlyBase ID	FBti0006473
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ark, Ecol\lacZ	Viability / fertility	viable
Causes allele(s)	Ark^k11502, Ecol\lacZ^Ark-k11502	Stock availability	3 publicly available
LINE ID	l(2)k11502
Genomic Location
Chromosomal location	2R ( 53E4 )	Sequence location	2R:12,907,578..12,907,578 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:12,907,578..12,907,578 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	53E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	53F1-53F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025966 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ark (Kanuka et al., 1999, BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999, Zhou et al., 1999, Arama et al., 2003)
Alleles and Phenotypes
Causes alleles	Ark^k11502 (Kanuka et al., 1999, Zhou et al., 1999, Kanuka et al., 2005, Kanuka et al., 2005, Zhou and Steller, 2003, Rodriguez et al., 2002) Ecol\lacZ^Ark-k11502 (BDGP Project Members, 1994-1999, Zhou et al., 1999, Arama et al., 2003)

Lethality References lethal \| embryonic stage \| recessive (Igaki et al., 2002) partially lethal - majority die \| pupal stage \| recessive (Kanuka et al., 1999) viable (Rodriguez et al., 1999)
Sterility References
Phenotype Manifest In
	dorsal closure embryo (Zhou et al., 1999) embryonic/larval brain (Kanuka et al., 1999) embryonic/larval digestive system (Minakhina and Steward, 2006) embryonic/larval salivary gland (Minakhina and Steward, 2006) embryonic brain (Zhou et al., 1999) embryonic head (Zhou et al., 1999) eo neuron \| supernumerary (Kanuka et al., 2005) eye photoreceptor cell (Kanuka et al., 1999) macrochaeta (Kanuka et al., 1999) macrochaeta \| supernumerary (Kanuka et al., 2005) melanotic mass (Minakhina and Steward, 2006) ommatidium (Kanuka et al., 1999) pigment cell (Kanuka et al., 1999) scutellar bristle \| ectopic (Kanuka et al., 1999) sensory mother cell \| supernumerary (Kanuka et al., 2005) thecogen cell \| supernumerary (Kanuka et al., 2005)
Detailed Description
Statement Reference Ark^k11502/Df(2R)BSC49 and Ark^k11502/Ark^P803-Δ15 third instar larvae show gut melanisation not accompanied by tissue overgrowth or hemocyte encapsulation. Homozygous third instar larvae develop multiple black spots along the gut and sometimes in the salivary glands. (Minakhina and Steward, 2006) 48% of Ark^k11502 mutant flies exhibit extra macrochaetae on the scutellum. Ark^k11502 mutants have one extra SOP cell in the scutellum region of the third instar wing disc. Analysis of the scutellum region of the pupal notum (24-30 h after pupal formation (APF)) shows that there is an extra neuron and thecogen present, which are derived from the extra SOP cell. (Kanuka et al., 2005) Unlike wild-type embyros UV irradition of Ark^k11502 homozygous pre-gastrula embryos at 50 mJ/cm² does not induce cell death. (Zhou and Steller, 2003) The hatch rate of homozygous Ark^k11502 embryos is not reduced if the embryos are injected with th dsRNA (produced by annealing th^cIa and th^a.cIa RNA). (Igaki et al., 2002) Mutants show only slight cell death defects in the embryo and barely detectable defects in adults. (Rodriguez et al., 2002) The eye phenotype caused by expression of rpr^GMR.PW is significantly suppressed by Ark^k11502. The eye phenotype caused by expression of W^GMR.PG is not affected by Ark^k11502. (Kanuka et al., 1999) The outer morphology of homozygous larvae and flies appears normal, except for adult dorsal bristles; extra bristles are often seen on the scutellum. Abnormal ommatidia with one extra photoreceptor cell are often seen. The morphology of the pigment cell layer is disorganised compared to the regular pattern seen in wild-type flies. A significant decrease in apoptotic cells is seen in stage 11-12 homozygous embryos compared to wild type. The brain hemispheres of third instar larvae are larger than normal and contain a markedly decreased number of apoptotic cells. (Kanuka et al., 1999) Homozygous stage 12/1 embryos contain fewer apoptotic cells in the head region that wild-type embryos. The phenotype is highly penetrant but some variation is seen. Embryos often show defects in head involution and the brain appears larger than normal. Suppresses the eye phenotype caused by Dcp-1^GMR.PS or Dcp-1^N.GMR. A strong synergism in cell ablation is seen when Dcp-1^GMR.PS and rpr^GMR.PW are coexpressed in the eye. This phenotype is not modified by Ark^k11502. (Zhou et al., 1999)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage \| early organism \| ubiquitous (Zhou et al., 1999) embryonic stage 10 -- 11 procephalic neurogenic region (Zhou et al., 1999) head mesoderm (Zhou et al., 1999) embryonic stage 11 organism \| segmentally repeated (Zhou et al., 1999) embryonic stage 11 -- 17 macrophage (Zhou et al., 1999)
Additional Information
Statement Reference A basal level of expression is observed all over the embryo. Elevated levels are observed in the procephalic region. Later expression is observed in a segmentally repeated pattern and in macrophages. (Zhou et al., 1999)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k11502 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	11041 y¹ w^67c23; P{lacW}Ark^k11502/CyO 23285 y¹ w^*; P{lacW}Ark^k11502 Ark⁸²/CyO, y⁺
Kyoto	111308 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Ark^k11502 /CyO y⁺
Linkouts
Comments
	Location 2R:12534936-12534937 confirmed by FlyBase alignment of dbGSS accession AQ025966 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) Insertion separable from lethal phenotype induced on same chromosome. (Rodriguez et al., 1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Ark^k11502 (FlyBase, 2005, FlyBase, 1992-, ) P{lacW}k11502 (FlyBase, 1992-) P{lacW}l(2)k11502^k11502 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 18 )
Research paper	Minakhina and Steward, 2006, Genetics 174(1): 253--263 Melanotic mutants in Drosophila: pathways and phenotypes. [FBrf0194475] Kanuka et al., 2005, EMBO J. 24(21): 3793--3806 Drosophila caspase transduces Shaggy/GSK-3beta kinase activity in neural precursor development. [FBrf0190361] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Arama et al., 2003, Dev. Cell 4(5): 687--697 Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila. [FBrf0158862] Zhou and Steller, 2003, Dev. Cell 4(4): 599--605 Distinct pathways mediate uv-induced apoptosis in Drosophila embryos. [FBrf0158860] Igaki et al., 2002, J. Biol. Chem. 277(26): 23103--23106 Down-regulation of DIAP1 triggers a novel Drosophila cell death pathway mediated by Dark and DRONC. [FBrf0149059] Rodriguez et al., 2002, EMBO J. 21(9): 2189--2197 Unrestrained caspase-dependent cell death caused by loss of Diap1 function requires the Drosophila Apaf-1 homolog, Dark. [FBrf0147000] Kanuka et al., 1999, Mol. Cell 4(5): 757--769 Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator. [FBrf0123073] Rodriguez et al., 1999, Nat. Cell Biol. 1(5): 272--279 Dark is a Drosophila homologue of Apaf-1/CED-4 and functions in an evolutionarily conserved death pathway. [FBrf0112020] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Zhou et al., 1999, Mol. Cell 4(5): 745--755 HAC-1, a Drosophila homolog of APAF-1 and CED-4 functions in developmental and radiation-induced apoptosis. [FBrf0123261] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Kanuka et al., 2005, EMBO J. 24(21): Drosophila caspase transduces Shaggy/GSK-3beta kinase activity in neural precursor development. [FBrf0191762]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Arkk11502 Insertion

Dmel\P{lacW}Ark^k11502 Insertion