FB2025_01 , released February 20, 2025
Insertion: Dmel\P{lacW}MESK2k10317
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General Information
Symbol
Dmel\P{lacW}MESK2k10317
Species
D. melanogaster
Name
FlyBase ID
FBti0006515
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k10317

Affected gene(s)
Ecol\lacZ, l(2)k10317, MESK2
Viability / fertility
Associated allele(s)
Ecol\lacZk10317, l(2)k10317k10317, MESK2k10317
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2R ( 57E8 )
Sequence Location
2R:21,502,124..21,502,124 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:21,502,124..21,502,124 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
57E8 (inferred by FlyBase from sequence location)
Cytological location (reported)
57E6-57E7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ025934
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k10317

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion. The insertion maps within the MESK2 transcription unit. However, since the insertion lies within a gene-rich region, the lethality can not yet be conclusively mapped to a single gene from these results. Thus l(2)k10317k10317 (representing the lethality of the chromosome) has been kept as a separate allele in FlyBase for now.

(Kahsai et al., 2016)

Location 2R:17009706-17009707 confirmed by FlyBase alignment of dbGSS accession AQ025934 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}MESK2k10317
P{lacW}l(2)k10317k10317
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (9)