Dmel\P{lacW}cora^k08713 Insertion

General Information
Symbol	Dmel\P{lacW}cora^k08713	Species	D. melanogaster
Name		FlyBase ID	FBti0006622
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	cora, Ecol\lacZ	Viability / fertility
Causes allele(s)	cora^k08713, Ecol\lacZ^cora-k08713	Stock availability	2 publicly available
LINE ID	l(2)k08713
Genomic Location
Chromosomal location	2R ( 56C4 )	Sequence location	2R:15,117,308..15,117,308 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:15,117,308..15,117,308 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	56C4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	56C1-56C2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034017 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	cora (BDGP Project Members, 1994-1999, Chen et al., 2005) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	cora^k08713 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Chen et al., 2005) Ecol\lacZ^cora-k08713 (BDGP Project Members, 1994-1999)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous cora^k08713, cora^k08713/cora⁵, and cora^k08713/Df(2R)BSC26 mutants show electrophysiologically detectable loss of glutamate receptors. Homozygous cora^k08713 mutants exhibit a glutamate-gated current amplitude that is approximately half that found in wild-type. sEJC (spontaneous excitatory junction currents) amplitudes, which reflect the number of functional glutamate receptors localised specifically to synapses, are reduced to approximately 80pA in cora^k08713 mutants, compared to approximately 154pA in wild-type. sEJC frequency is reduced in cora^k08713 mutants, although not to a significant level. sEJC decay time is significantly reduced in cora^k08713 mutants, consistent with a specific loss of A-type receptors. The single glutamate receptor channel amplitude is similar in both control and cora^k08713 mutants. Homozygous cora^k08713 mutants exhibit an average cluster size of A-type glutamate receptors that is reduced to approximately one-half of the wild-type size. The cluster size of B-type glutamate receptors, however, is not reduced in cora^k08713 mutants compared with controls. While in wild-type dynamic filamentous actin is required for maintenance of postsynaptic function, in cora^k08713 mutants this requirement is abolished, as exemplified by treatment with latrunculin A or cofilin having no effect. Treatment with latrunculin A has no effect on the size of A-type receptor clusters in cora^k08713 mutants (compared to a reduction in wild-type). More specifically, treatment with latrunculin A has no effect on the size of GluRIIA receptor clusters in cora^k08713 mutants (compared to a reduction in wild-type). (Chen et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08713 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10801 y¹ w^67c23; P{lacW}cora^k08713/CyO
Kyoto	111245 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}cora^k08713 /CyO y⁺
Linkouts
Comments
	Location 2R:14744666-14744667 confirmed by FlyBase alignment of dbGSS accession AQ034017 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}cora^k08713 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(2)k08713^k08713 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{w^'+mC'=lacW}cora^k08713 (Chen et al., 2005)
Secondary FlyBase IDs

References ( 10 )
Research paper	Chen et al., 2005, J. Neurosci. 25(28): 6667--6675 The 4.1 protein coracle mediates subunit-selective anchoring of Drosophila glutamate receptors to the postsynaptic actin cytoskeleton. [FBrf0187898] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Deal-Herr and Cook, 2002.12.17, Isolation and characterization of Df(2R)BSC26. Isolation and characterization of Df(2R)BSC26. [FBrf0155382]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}corak08713 Insertion

Dmel\P{lacW}cora^k08713 Insertion