Dmel\P{lacW}k08617 Insertion

General Information
Symbol	Dmel\P{lacW}k08617	Species	D. melanogaster
Name		FlyBase ID	FBti0006624
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	bowl, Ecol\lacZ	Viability / fertility
Causes allele(s)	Ecol\lacZ^k08617	Stock availability	2 publicly available
LINE ID	l(2)k08617
Genomic Location
Chromosomal location	2L ( 24C3 )	Sequence location	2L:3,771,702..3,771,702 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:3,771,702..3,771,702 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	24C3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	24C1-24C2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025865 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	bowl (Hao et al., 2003, BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999, Hao et al., 2003)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^k08617 (BDGP Project Members, 1994-1999, Hao et al., 2003)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference larval stage ventral thoracic disc \| restricted (Hao et al., 2003) pupal stage leg \| segmentally repeated (Hao et al., 2003)
Additional Information
Statement Reference Larval expression is observed in the center of the leg discs. Pupal expression is observed in a broad stripe in each developing leg segment, including tarsal segments 1-4. (Hao et al., 2003)

Marker for	ventral thoracic disc \| restricted pupal leg (Hao et al., 2003)
Reflects expression of	bowl (Hao et al., 2003)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08617 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10794 y¹ w^67c23; P{lacW}k08617, l(2)k08617^k08617/CyO
Kyoto	111243 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}k08617 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:3771701-3771702 determined by FlyBase alignment of dbGSS accession AQ025865 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	bowl-LacZ (Hao et al., 2003) bowl-lacZ (Hao et al., 2003) P{lacW}bowl^k08617 (FlyBase, 1992-) P{lacW}k08617 (FlyBase, 1992-) P{lacW}l(2)k08617^k08617 (FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Hao et al., 2003, Dev. Biol. 263(2): 282--295 The odd-skipped family of zinc finger genes promotes Drosophila leg segmentation. [FBrf0167461] Johansen et al., 2003, Mech. Dev. 120(10): 1139--1151 The Drm-Bowl-Lin relief-of-repression hierarchy controls fore- and hindgut patterning and morphogenesis. [FBrf0167839] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Lengyel, 2003.6.4, P{Mae-UAS.6.11}bowl<up>RBG</up>. P{Mae-UAS.6.11}bowl^RBG. [FBrf0159870]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]