Dmel\P{lacW}Rab5^k08232 Insertion

General Information
Symbol	Dmel\P{lacW}Rab5^k08232	Species	D. melanogaster
Name		FlyBase ID	FBti0006638
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Rab5	Viability / fertility
Causes allele(s)	Ecol\lacZ^Rab5-k08232, Rab5^k08232	Stock availability	2 publicly available
LINE ID	l(2)k08232
Genomic Location
Chromosomal location	2L ( 22E1 )	Sequence location	2L:2,364,870..2,364,870 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:2,364,870..2,364,870 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	22E1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	22E1--22E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 22E1-22E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025862 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Rab5 (BDGP Project Members, 1994-1999, Chen et al., 2008)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Rab5-k08232 (BDGP Project Members, 1994-1999) Rab5^k08232 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Misra, 2000.8.25, Deal et al., 2003.7.22, Chen et al., 2008)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999) semi-lethal \| early third instar larval stage (Wucherpfennig et al., 2003) semi-lethal \| second instar larval stage (Wucherpfennig et al., 2003)
Sterility References fertile \| poor (Wucherpfennig et al., 2003)
Phenotype Manifest In
	crystal cell (Milchanowski et al., 2004) synaptic vesicle & larval neuromuscular junction (Wucherpfennig et al., 2003)
Detailed Description
Statement Reference The average number of crystal cells per embryo is reduced (to 11.4) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). (Milchanowski et al., 2004) Homozygous Rab5^k08232 animals die during late second and early third instar larval stages with only a light locomotion phenotype. Consistent with a mild phenotype, both synaptic uptake and transmission rates to the muscles are normal. If raised under `intensive care\' conditions, very few Rab5^k08232 larvae develop into adult flies, which display a flightless phenotype. (Wucherpfennig et al., 2003) The Rab5^k08232 lethality and phenotype are caused by reduced Rab5 levels in the nervous system as they can be rescued by restricted expression of Rab5^{Scer\UAS.T:Avic\GFP-EGFP}, under the regulation of Scer\GAL4^elav-C155 in the nervous system. Rab5^+tWa rescues the lethality due to Rab5^k08232. (Wucherpfennig et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k08232 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10786 y¹ w^67c23; P{lacW}Rab5^k08232/CyO
Kyoto	111239 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Rab5^k08232 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:2364870-2364871 confirmed by FlyBase alignment of dbGSS accession AQ025862 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005) The P{PZ}00231 insertion maps 115bp away from the P{lacW}Rab5^k08232 insertion.The P{lacW}Rab5^k08232 insertion maps 236bp upstream of BcDNA:GH09817. (Misra, 2000.8.25)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k08232^k08232 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}Rab5^k08232 (FlyBase, 2005, FlyBase, 1992-, Chen et al., 2008)
Secondary FlyBase IDs

References ( 12 )
Research paper	Chen et al., 2008, Genome Res. 18(1): 123--136 Identification of novel modulators of mitochondrial function by a genome-wide RNAi screen in Drosophila melanogaster. [FBrf0202125] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Milchanowski et al., 2004, Genetics 168(1): 325--339 Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297] Wucherpfennig et al., 2003, J. Cell Biol. 161(3): 609--624 Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release. [FBrf0159035] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Deal et al., 2003.7.22, Isolation and characterization of Df(2L)BSC37. Isolation and characterization of Df(2L)BSC37. [FBrf0161589] Misra, 2000.8.25, nc5 report, second installment. nc5 report, second installment. [FBrf0131112]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Rab5k08232 Insertion

Dmel\P{lacW}Rab5^k08232 Insertion