FB2025_01 , released February 20, 2025
Insertion: Dmel\P{lacW}Rab5k08232
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General Information
Symbol
Dmel\P{lacW}Rab5k08232
Species
D. melanogaster
Name
FlyBase ID
FBti0006638
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k08232

Affected gene(s)
Ecol\lacZ, Rab5
Viability / fertility
Associated allele(s)
Ecol\lacZRab5-k08232, Rab5k08232
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2L ( 22E1 )
Sequence Location
2L:2,364,870..2,364,870 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:2,364,870..2,364,870 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
22E1 (inferred by FlyBase from sequence location)
Cytological location (reported)
22E1--22E2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
22E1-22E2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ025862
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Rab5
(Chen et al., 2008, BDGP Project Members, 1994-1999)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k08232

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:2364870-2364871 confirmed by FlyBase alignment of dbGSS accession AQ025862 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

The P{PZ}00231 insertion maps 115bp away from the P{lacW}Rab5k08232 insertion.The P{lacW}Rab5k08232 insertion maps 236bp upstream of BcDNA:GH09817.

(Misra, 2000.8.25)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}Rab5k08232
(Chen et al., 2008, FlyBase, 2005, FlyBase, 1992-)
P{lacW}l(2)k08232k08232
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (14)