A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}wcdk07824 Insertion

General Information
Symbol Dmel\P{lacW}wcdk07824 Species D. melanogaster
Name FlyBase ID FBti0006671
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, wcd Viability / fertility
Causes allele(s) Ecol\lacZk07824, wcdk07824 Stock availability 2 publicly available
LINE ID l(2)k07824
Genomic Location
Chromosomal location 2R ( 53C1 ) Sequence location 2R:12,234,831..12,234,831 [+]
Map ( GBrowse ) detailed view FBti0141119 FBti0039341 FBti0103879 FBti0103390 FBti0110229 FBti0112987 FBti0075093 FBti0112927 FBti0006671 FBti0106688 FBti0102658 FBti0103560 FBti0103297 FBti0103296
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:12,234,831..12,234,831 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
53C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
53C1-53C4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ034005
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
wcd
(BDGP Project Members, 1994-1999, Fichelson et al., 2009, Fichelson et al., 2009)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZk07824
(BDGP Project Members, 1994-1999)
wcdk07824
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Fichelson et al., 2009, Fichelson et al., 2009)
Lethality
References
lethal
(Fichelson et al., 2009)
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
female germline stem cell | germline clone
(Fichelson et al., 2009)
ganglion cell | somatic clone
(Fichelson et al., 2009)
neuroblast | somatic clone
(Fichelson et al., 2009)
hide Detailed Description
Statement
Reference
The rate of loss of homozygous wcd[k07824] germline stem cell clones (GSCs) in the ovary as females age is much more pronounced than the rate of loss of wild-type GSCs as females age. Homozygous ventral nerve cord neuroblast clones produce fewer ganglion cells than wild-type neuroblast clones. The mutant neuroblasts are on average half the size of wild-type neuroblasts.
(Fichelson et al., 2009)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k07824
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10686
y1 w67c23; P{lacW}wcdk07824/CyO
Kyoto
111224
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}wcdk07824 /CyO y+
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hide Comments
Location 2R:11862189-11862190 confirmed by FlyBase alignment of dbGSS accession AQ034005 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k07824k07824
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}wcdk07824
 
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Fichelson et al., 2009, Nat. Cell Biol. 11(6): 685--693
Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila. [FBrf0210070]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Fichelson et al., 2009, Nat. Cell Biol. 11(6):
Online methods. [FBrf0209977]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]