A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}mblk07110 Insertion

General Information
Symbol Dmel\P{lacW}mblk07110 Species D. melanogaster
Name FlyBase ID FBti0006712
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, mbl Viability / fertility viable
Causes allele(s) Ecol\lacZmbl-k07110, mblk07110 Stock availability 1 publicly available
LINE ID l(2)k07110
Genomic Location
Chromosomal location 2R ( 54B1 ) Sequence location 2R:13,152,995..13,152,995 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0029575 FBti0108962 FBti0151842 FBti0006712 FBti0006845 FBti0005325 FBti0039834 FBti0025739 FBti0036423 FBti0112668 FBti0034709 FBti0108184 FBti0034839 FBti0034439 FBti0102732 FBti0007501 FBti0103059 FBti0104081 FBti0106784 FBti0066052 FBti0047815 FBti0045604 FBti0045309 FBti0109978 FBti0006902 FBti0046657
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:13,152,995..13,152,995 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
54B1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
54B1-54B2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AB036794
(FlyBase, 1992-)
AQ025815
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
mbl
(BDGP Project Members, 1994-1999, Juni and Yamamoto, 2009)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZmbl-k07110
(BDGP Project Members, 1994-1999)
mblk07110
(Spradling et al., 1999, Spradling, 1999.12.9, Misra, 2000.11.18, Juni and Yamamoto, 2009)
Lethality
References
partially lethal - majority die
(Juni and Yamamoto, 2009)
viable
(Spradling et al., 1999, BDGP Project Members, 1994-1999, Juni and Yamamoto, 2009)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Fails to complement the reduced female sexual receptivity phenotype of mbl[chst].
(Juni and Yamamoto, 2009)
mbl[chst]/mbl[k07110] females show reduced sexual receptivity compared to controls.
(Juni and Yamamoto, 2009)
No obvious phenotype.
(Spradling et al., 1999)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k07110
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Kyoto
102524
y1 w67c23 ; P{lacW}mblk07110 / CyO
hide Linkouts
hide Comments
Location 2R:12780353-12780354 confirmed by FlyBase alignment of dbGSS accession AQ025815 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
Insertion separable from lethal originally isolated on the same chromosome.
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Separable from l(2)k07110k07110.
(BDGP Project Members, 1994-1999)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k07110k07110
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}mblk07110
(FlyBase, 2005, FlyBase, 1992-, )
P{lacW}mmk07110
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Juni and Yamamoto, 2009, J. Neurogenet. 23(3): 329--340
Genetic analysis of chaste, a new mutation of Drosophila melanogaster characterized by extremely low female sexual receptivity. [FBrf0209818]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Misra, 2000.11.18, nc5 report, third installment.
nc5 report, third installment. [FBrf0132131]
Spradling, 1999.12.9, Helping FlyBase with P-element paper.
Helping FlyBase with P-element paper. [FBrf0125024]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]