Dmel\P{lacW}Fpps^k06103 Insertion

General Information
Symbol	Dmel\P{lacW}Fpps^k06103	Species	D. melanogaster
Name		FlyBase ID	FBti0006758
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Fpps	Viability / fertility
Causes allele(s)	Ecol\lacZ^Fpps-k06103, Fpps^k06103	Stock availability	2 publicly available
LINE ID	l(2)k06103
Genomic Location
Chromosomal location	2R ( 47F1 )	Sequence location	2R:7,193,655..7,193,655 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:7,193,655..7,193,655 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	47F1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	47F1-47F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034152 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Milchanowski et al., 2004) Fpps (BDGP Project Members, 1994-1999, Cook et al., 2012)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Fpps-k06103 (BDGP Project Members, 1994-1999, Milchanowski et al., 2004) Fpps^k06103 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Santos and Lehmann, 2004, Beaton, 2000.1.31, Chung et al., 2001, Cook et al., 2012)

Lethality References lethal (Cook et al., 2012) lethal \| recessive (Beaton, 2000.1.31, BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	crystal cell (Milchanowski et al., 2004) pole cell (Santos and Lehmann, 2004) pole cell \| germline clone \| rescuable maternal effect (Santos and Lehmann, 2004)
Detailed Description
Statement Reference Fpps[k06103] heterozygous mutants do not display a degenerative nervous system phenotype, with only the occasional small vacuole forming in these mutants. (Cook et al., 2012) A Fpps[k06103] heterozygous background significantly suppresses the degenerative nervous sytem phenotype found in 5 day old SNF4Aγ[loe] mutants. These flies exhibit a reduction in the area of vacuoles in the optic system, from approximately 163 υm[2] to 25υm[2] in a Fpps[k06103]/+ background. There is a similar reduction in the number of vacuoles present, confirming an involvement of the isoprenoid pathway in the degenerative phenotype of SNF4Aγ[loe] flies. (Cook et al., 2012) The average number of crystal cells per embryo is reduced (to 10.5) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average). (Milchanowski et al., 2004) Germ cell migration is severely defective in Fpps^k06103; qm^L14.4 double homozygous embryos. About 40% of Fpps^k06103; Hmgcr⁰¹¹⁵² double homozygous embryoshave severe patterning defects. However, in the remaining 60% the mesoderm appears correctly patterned but germ cell migration is severely defective (on average 14 germ cells remain on the gut), an enhancement over the germ cell migration phenotype seen in either single mutant. (Santos and Lehmann, 2004) In embryos homozygous Fpps^k06103, a small number of germ cells (4 on average) fail to migrate correctly toward the mesoderm and instead remain associated with the dorsal side of the posterior midgut. This phenotype is stronger in embryos both maternally and zygotically homozygous for Fpps^k06103: 13 germ cells, on average, fail to migrate into the mesoderm. (Santos and Lehmann, 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k06103 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10604 y¹ w^67c23; P{lacW}Fpps^k06103/CyO
Kyoto	111189 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Fpps^k06103 /CyO y⁺
Linkouts
Comments
	Location 2R:6822057-6822058 confirmed by FlyBase alignment of dbGSS accession AQ034152 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	fpps^k06103 (Cook et al., 2012) P{lacW}Fpps^k06103 (FlyBase, 2005, FlyBase, 1992-, ) P{lacW}l(2)k06103^k06103 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Cook et al., 2012, PLoS ONE 7(9): e44440 Increased RhoA Prenylation in the loechrig (loe) Mutant Leads to Progressive Neurodegeneration. [FBrf0219441] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Milchanowski et al., 2004, Genetics 168(1): 325--339 Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297] Santos and Lehmann, 2004, Dev. Cell 6(2): 283--293 Isoprenoids control germ cell migration downstream of HMGCoA reductase. [FBrf0174539] Chung et al., 2001, Neuron 29(2): 415--428 nompA encodes a PNS-specific, ZP domain protein required to connect mechanosensory dendrites to sensory structures. [FBrf0134806] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Beaton, 2000.1.31, BDGP P element Update. BDGP P element Update. [FBrf0126832] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Fppsk06103 Insertion

Dmel\P{lacW}Fpps^k06103 Insertion