A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Dmel\P{lacW}mei-W68k05603 Insertion

General Information
Symbol Dmel\P{lacW}mei-W68k05603 Species D. melanogaster
Name FlyBase ID FBti0006787
Feature type transposable_element_insertion_site Created / Updated 1998-01-03/1998-11-12
Description
Inserted element P{lacW} Localized function
Affected gene(s) mei-W68, par-1 Expression data
Causes allele(s) par-1k05603, mei-W68k05603 Viability / fertility
LINE ID l(2)k05603 Stock availability
Genomic Location
Chromosomal location 2R ( 56D9 ) Sequence location 2R:15,344,455..15,344,462 [+]
Map ( GBrowse ) detailed view FBti0025856 FBti0076010 FBti0028719 FBti0006925 FBti0071164 FBti0067070 FBti0011303 FBti0046521 FBti0056388 FBti0099882 FBti0067112 FBti0034062 FBti0057703 FBti0036930 FBti0023596 FBti0059584 FBti0028718 FBti0025665 FBti0039941 FBti0007774 FBti0059583 FBti0006787 FBti0072159 FBti0016284 FBti0045077 FBti0014543 FBti0029327 FBti0070158 FBti0006751 FBti0026139 FBti0046662 FBti0056756 FBti0046836
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:15,344,455..15,344,462 [+]
(McKim and Hayashi-Hagihara, 1998, BDGP Project Members, 1994-1999)
2R:15,344,452..15,344,452 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
56D9 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
56D8-56D10 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Chromosome in situ
hide Sequence Data
Flanking sequence
AQ025763
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role 		 
Size 10.691Kb
Associated alleles
w+mC
Ecol\lacZP\T.W
Molecular map
Transposon class
Element type
hide Affected Gene(s)
Insertion within gene
Insertion may
affect gene
mei-W68
(BDGP Project Members, 1994-1999)
par-1
(Shulman et al., 2000, Shulman and McKim, 2000.6.20, Tomancak et al., 2000)
Orientation
(relative to gene)
Gene
Orientation
References
 
 
 
hide Alleles and Phenotypes
Causes alleles
par-1k05603
(Shulman et al., 2000, Shulman and McKim, 2000.6.20, Tomancak et al., 2000)
mei-W68k05603
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Iida and Lilly, 2004, Shulman and McKim, 2000.6.20, McKim and Hayashi-Hagihara, 1998)
Lethality
References
viable
(Shulman and McKim, 2000.6.20)
lethal | recessive
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
female fertile
(Tomancak et al., 2000)
hide Phenotype Manifest In
embryonic abdominal segment
(Tomancak et al., 2000)
embryo | posterior
(Tomancak et al., 2000)
pole cell
(Riechmann et al., 2002)
germ cell
(Riechmann et al., 2002)
hide Detailed Description
Statement
Reference
Embryos derived from par-1k06323/par-1k05603 ; osk1/+ females have an average of 3 pole cells/embryo (compared to 33 in the wild-type), and 42% of the embryos lack a germline.
(Riechmann et al., 2002)
20% of embryos derived from par-1k05603/par-1k06323 females die, showing defects which range from disturbances in posterior patterning to complete absence of abdominal segments.
(Tomancak et al., 2000)
Embryos derived from par-1k06323/par-1k05603 females have an average of 19 pole cells/embryo (compared to 33 in the wild-type), and 12% of the embryos lack a germline.
(Riechmann et al., 2002)
The majority of mei-W68[1]/mei-W68[k05603] eggs (94%) show a wild-type number of dorsal appendages.
(Klattenhoff et al., 2007)
The fused dorsal appendage phenotype of armi[72.1]/armi[1] eggs is not suppressed in mei-W68[1]/mei-W68[k05603]; armi[72.1]/armi[1] eggs.
(Klattenhoff et al., 2007)
Transformation of oocytes to nurse cells in mio2/mio2 or mio2/mio1 females is partially suppressed by mei-W68k05603/+.
(Iida and Lilly, 2004)
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k05603
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 3 )
Kyoto
102395
Df(1)w67c23, y1 ; P{lacW}mei-W68k05603 / CyO
108858
y1 w67c23; P{lacW}mei-W68k05603/CyO
Bloomington
10574
y1 w67c23; P{lacW}mei-W68k05603 par-1k05603/CyO
hide Linkouts
hide Comments
Location 2R:14971810-14971811 confirmed by FlyBase alignment of dbGSS accession AQ025763 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
8bp insertion associated host repeat
(McKim and Hayashi-Hagihara, 1998, BDGP Project Members, 1994-1999)
P5' to P3' relative to + orientation of reference sequence
(McKim and Hayashi-Hagihara, 1998, BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k05603k05603
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}mei-W68k05603
(FlyBase, 2005, FlyBase, 1992-, McKim and Hayashi-Hagihara, 1998, BDGP Project Members, 1994-1999)
Secondary FlyBase IDs
    hide References ( 14 )
    Research paper
    Klattenhoff et al., 2007, Dev. Cell 12(1): 45--55
    Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. [FBrf0192439]
    Bellen et al., 2004, Genetics 167(2): 761--781
    The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
    Iida and Lilly, 2004, Development 131(5): 1029--1039
    missing oocyte encodes a highly conserved nuclear protein required for the maintenance of the meiotic cycle and oocyte identity in Drosophila. [FBrf0174551]
    Riechmann et al., 2002, Nature Cell Biol. 4(5): 337--342
    Par-1 regulates stability of the posterior determinant Oskar by phosphorylation. [FBrf0149128]
    Vaccari and Ephrussi, 2002, Curr. Biol. 12(17): 1524--1528
    The fusome and microtubules enrich Par-1 in the oocyte, where it effects polarization in conjunction with Par-3, BicD, Egl, and dynein. [FBrf0151842]
    Shulman et al., 2000, Cell 101(4): 377--388
    The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole. [FBrf0128644]
    Tomancak et al., 2000, Nature Cell Biol. 2(7): 458--460
    A Drosophila melanogaster homologue of Caenorhabditis elegans par-1 acts at an early step in embryonic-axis formation. [FBrf0128666]
    Spradling et al., 1999, Genetics 153(1): 135--177
    The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
    McKim and Hayashi-Hagihara, 1998, Genes Dev. 12(18): 2932--2942
    mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that the mechanism for initiating meiotic recombination is conserved. [FBrf0104768]
    Personal communication to FlyBase
    Shulman and McKim, 2000.6.20, l(2)k05603 and EP(2)1144.
    l(2)k05603 and EP(2)1144. [FBrf0129205]
    FlyBase analysis
    FlyBase, 2005, Assessment of transgenic construct insertion sites.
    Assessment of transgenic construct insertion sites. [FBrf0184339]
    FlyBase, 1992-, FlyBase curation
    FlyBase curation. [FBrf0105495]
    Computer file
    Gene Disruption Project members, 2001-, [title not yet available]
    [title not yet available] [FBrf0132177]
    BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
    Berkeley Drosophila Genome Project. [FBrf0067338]