Dmel\P{lacW}RanBPM^k05201 Insertion

General Information
Symbol	Dmel\P{lacW}RanBPM^k05201	Species	D. melanogaster
Name		FlyBase ID	FBti0006813
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, RanBPM	Viability / fertility
Causes allele(s)	Ecol\lacZ^{RanBPM-k05201}, RanBPM^k05201	Stock availability	1 publicly available
LINE ID	l(2)k05201
Genomic Location
Chromosomal location	2R ( 47A7 )	Sequence location	2R:6,318,098..6,318,098 [-]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:6,318,098..6,318,098 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	47A7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	47A3-47A5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025753 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) RanBPM (BDGP Project Members, 1994-1999, Scantlebury et al., 2010)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{RanBPM-k05201} (BDGP Project Members, 1994-1999) RanBPM^k05201 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Scantlebury et al., 2010)

Lethality References lethal \| larval stage (Scantlebury et al., 2010) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Expression of RanBPM[long.Scer\UAS] under the control of Scer\GAL4[elav.PLu] effectively rescues the response to light of RanBPM[k05201] mutant larvae. The overall locomotion of the mutant larvae is also improved. The feeding defects of RanBPM[k05201] mutant larvae are rescued by expression of RanBPM[long.Scer\UAS] under the control of Scer\GAL4[elav.PLu], Scer\GAL4[Mef2.PR] or Scer\GAL4[Mef2.247]. Expression of RanBPM[long.Scer\UAS] under the control of Scer\GAL4[Mef2.247] at 29[o]C significantly rescues the response to light of RanBPM[k05201] mutant larvae. The overall locomotion of the mutant larvae is also improved. Expression of RanBPM[S.Scer\UAS] under the control of Scer\GAL4[elav.PLu] effectively rescues the response to light of RanBPM[k05201] mutant larvae. The feeding defects of RanBPM[k05201] mutant larvae are rescued by expression of RanBPM[S.Scer\UAS] under the control of Scer\GAL4[elav.PLu], Scer\GAL4[Mef2.PR], Scer\GAL4[386Y] or Scer\GAL4[Mef2.247]. Expression of RanBPM[S.Scer\UAS] under the control of Scer\GAL4[Mef2.247] at 29[o]C significantly rescues the response to light of RanBPM[k05201] mutant larvae. Expression of RanBPM[S.Scer\UAS] under the control of Scer\GAL4[Mef2.PR] significantly rescues the response to light of RanBPM[k05201] mutant larvae. (Scantlebury et al., 2010) Homozygous third instar larvae show a significantly lower response index (response index = (path length in dark - path length in light)/total path length during the assay) in an on/off light avoidance assay than control larvae. The mutant larvae show reduced locomotion and are uncoordinated (as seen by the equally high degree of direction change during the lights on and off pulses). RanBPM[k05201]/RanBPM[ts7] and RanBPM[k05201]/RanBPM[s135] third instar larvae show a significantly lower response index (response index = (path length in dark - path length in light)/total path length during the assay) in an on/off light avoidance assay than control larvae. Mutant larvae show reduced locomotion in constant darkness conditions. Homozygous larvae are smaller than control larvae of the same age from the early third instar stage onwards. A smaller fraction of homozygous early foraging third instar larvae that have been placed on a drop of yeast paste containing food dye after a 2 hour starvation period show food dye in the gut after 30 minutes on the yeast paste. If the feeding period after starvation is extended to 1.5 hours, the fraction of mutant larvae containing food dye in the gut at the end of the feeding period does not increase. In addition, a large fraction of the mutant larvae are found to have left the yeast drop (in contrast to wild-type larvae which mostly remain in the yeast drop). A smaller fraction of homozygous early foraging third instar larvae than control larvae move into and remain within a yeast drop if they are placed on medium outside a centrally located yeast drop containing food dye after a 2 hour starvation period and then allowed to feed for 1.5 hours (the control larvae largely move into and remain in the yeast drop in this assay). In addition, a lower proportion of the mutant larvae show food dye in the gut than control larvae at the end of the feeding period. (Scantlebury et al., 2010) The increased number of branches and of boutons at the neuromuscular junction of Fmr1[EP3517] and Fmr1[Δ50M] larvae is significantly suppressed by RanBPM[k05201]/+. (Scantlebury et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k05201 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10564 y¹ w^67c23; P{lacW}RanBPM^k05201/CyO
Linkouts
Comments
	Location 2R:5942405-5942406 determined by FlyBase alignment of dbGSS accession AQ025753 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k05201^k05201 (FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}RanBPM^k05201
Secondary FlyBase IDs

References ( 8 )
Research paper	Scantlebury et al., 2010, PLoS ONE 5(5): e10652 The Drosophila Gene RanBPM Functions in the Mushroom Body to Regulate Larval Behavior. [FBrf0210838] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}RanBPMk05201 Insertion

Dmel\P{lacW}RanBPM^k05201 Insertion