Dmel\P{lacW}shot^k03010 Insertion

General Information
Symbol	Dmel\P{lacW}shot^k03010	Species	D. melanogaster
Name		FlyBase ID	FBti0006877
Feature type	transposable_element_insertion_site	Created / Updated	1998-01-03/1999-05-09
Description
Inserted element	P{lacW}	Localized function
Affected gene(s)	shot	Expression data
Causes allele(s)	shot^k03010	Viability / fertility
LINE ID	l(2)k03010	Stock availability
Genomic Location
Chromosomal location	2R ( 50C9 )	Sequence location	2R:9,807,308..9,807,308 [+]
Map ( GBrowse )
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:9,807,308..9,807,308 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	50C9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	50C9-50C10 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Chromosome in situ
Sequence Data
Flanking sequence	AQ034139 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role
Size	10.691Kb
Associated alleles	w^+mC Ecol\lacZ^P\T.W
Molecular map
Transposon class
Element type
Affected Gene(s)
Insertion within gene
Insertion may affect gene	shot (BDGP Project Members, 1994-1999)
Orientation (relative to gene)	Gene Orientation References
Alleles and Phenotypes
Causes alleles	shot^k03010 (BDGP Project Members, 1994-1999, Lee et al., 2000, Strumpf and Volk, 1998, Gregory and Brown, 1998, Spradling et al., 1999)

Lethality References lethal \| recessive (Gregory and Brown, 1998, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	dendrite & embryo \| dorsal (Gao et al., 1999) embryonic peripheral nervous system \| dorsal (Gao et al., 1999) chordotonal organ & embryo \| lateral (Lee et al., 2000) extended germ band embryo (Gregory and Brown, 1998) dorsal closure embryo (Gregory and Brown, 1998)
Detailed Description
Statement Reference The lateral chordotonal sensory axons arrest at variable stages before reaching the central nervous system (CNS); 27% extend towards the intersegmental nerve (ISN) and stall or fail to reach the ISN, 55% travel a significant distance within the ISN, 18% reach the CNS but do not penetrate it, and none of the axons enter the CNS and form arborisations. (Lee et al., 2000) When homozygous germ-line clones are made, no defect is seen in oocyte determination. (Roper and Brown, 2004) Germ band retraction and head involution fail in approximately 15% of homozygous embryos. (Gregory and Brown, 1998) In the dorsal cluster of embryonic dendrites of shot³⁰/shot^k03010 embryos, the number of anterior lateral branches is reduced from about 10 to 4 per hemisegment, whereas more branches extend dorsally and appear to contact each other more extensively. (Gao et al., 1999)
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k03010 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10522 y¹ w^67c23; P{lacW}shot^k03010/CyO
Kyoto	102250 Df(1)w67c23, y¹ ; P{lacW}shot^k03010 / CyO
Linkouts
Comments
	Location 2R:9435710-9435711 confirmed by FlyBase alignment of dbGSS accession AQ034139 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k03010^k03010 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}shot^k03010 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 14 )
Research paper	Jones, 2005, Genetics 169(4): 2075--2086 RacGap50C negatively regulates wingless pathway activity during Drosophila embryonic development. [FBrf0188508] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Fuss et al., 2004, Development 131(7): 1587--1595 Cell movements controlled by the Notch signalling cascade during foregut development in Drosophila. [FBrf0174561] Roper and Brown, 2004, Curr. Biol. 14(2): 99--110 A Spectraplakin Is Enriched on the Fusome and Organizes Microtubules during Oocyte Specification in Drosophila. [FBrf0167424] Subramanian et al., 2003, Curr. Biol. 13(13): 1086--1095 Shortstop recruits EB1/APC1 and promotes microtubule assembly at the muscle-tendon junction. [FBrf0160988] Lee et al., 2000, J. Neurosci. 20(3): 1096--1108 short stop is allelic to kakapo, and encodes rod-like cytoskeletal-associated proteins required for axon extension. [FBrf0125142] Gao et al., 1999, Genes Dev. 13(19): 2549--2561 Genes regulating dendritic outgrowth, branching, and routing in Drosophila. [FBrf0111874] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Gregory and Brown, 1998, J. Cell Biol. 143(5): 1271--1282 kakapo, a gene required for adhesion between and within cell layers in Drosophila, encodes a large cytoskeletal linker protein related to plectin and dystrophin. [FBrf0105832] Strumpf and Volk, 1998, J. Cell Biol. 143(5): 1259--1270 Kakapo, a novel cytoskeletal-associated protein is essential for the restricted localization of the neuregulin-like factor, vein, at the muscle-tendon junction site. [FBrf0105940]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}shotk03010 Insertion

Dmel\P{lacW}shot^k03010 Insertion