Dmel\P{lacW}lolal^k02512 Insertion

General Information
Symbol	Dmel\P{lacW}lolal^k02512	Species	D. melanogaster
Name		FlyBase ID	FBti0006889
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, lolal	Viability / fertility
Causes allele(s)	Ecol\lacZ^lolal-k02512, lolal^k02512	Stock availability	2 publicly available
LINE ID	l(2)k02512
Genomic Location
Chromosomal location	2R ( 55B9 )	Sequence location	2R:14,044,444..14,044,444 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:14,044,444..14,044,444 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	55B9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	55B5-55B10 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034138 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Mishra et al., 2003) lolal (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^lolal-k02512 (BDGP Project Members, 1994-1999, Mishra et al., 2003) lolal^k02512 (BDGP Project Members, 1994-1999, Mishra et al., 2003, Spradling et al., 1999, Roch et al., 1998)

Lethality References lethal \| first instar larval stage \| recessive (Faucheux et al., 2003) lethal \| recessive (Spradling et al., 1999, Roch et al., 1998)
Sterility References
Phenotype Manifest In
	dorsal trunk primordium (Kerman et al., 2008) presumptive embryonic salivary gland (Kerman et al., 2008) sex comb \| ectopic (Faucheux et al., 2003)
Detailed Description
Statement Reference The developing salivary glands in lolal[k02512] mutant animals fail to turn and migrate properly. In addition, the dorsal trunks of the trachea exhibit only partial extension at stage 14, but relatively normal extension of dorsal and visceral branches occurs. (Kerman et al., 2008) lolal[k02512] mutants show a higher proportion of salivary gland defects in combination with rib[1]/+. In these animals the salivary glands fail to turn and migrate properly. However, tracheal dorsal trunk defects are not enhanced in these animals at stage 14. (Kerman et al., 2008) lolal^k02512 enhances the ph-p⁴¹⁰ esc-like phenotype, consisting of homeotic transformation of mesothoracic or metathoracic legs towards prothoracic legs due to the deregulation of Scr. Mutants heterozygous for lolal^k02512, when also heterozygous for ph-p⁴¹⁰, Pc¹⁶, Pc¹, Pc³ or Pc¹⁵, exhibit a contrabithorax phenotype, which is a wing-to-haltere transformation due to ectopic expression of Ubx in the posterior part of the wing disc, often resulting in curvature of the wing. This phenotype is further enhanced in the viable lolal^k02512/lolal^311a heteroallelic combinations, when also heterozygous for ph-p⁴¹⁰, Pc¹⁶, Pc¹, Pc³ or Pc¹⁵. lolal^k02512 ; Trl^13C heterozygous flies are viable and do not show any visible phenotype. Approximately 4% of lolal^k02512/+; Trl^13C/Trl^13C flies hatch. Adding at least one copy of the Hsp83^GAGA519 or Hsp83^GAGA581 transgenes allows the recovery of a much higher frequency of homozygous Trl^13C escapers that are also heterozygous for lolal^k02512 (up to approximately 80% when four copies of the transgene are added). Double heterozygous lolal^k02512 Ubx¹³⁰ flies do not show significant increase in the expressivity of the Ubx phenotype. lolal^k02512/+; Trl^13C/Ubx¹³⁰ flies display a higher frequency (18%) of strong haltere-to-wing transformations compared to Trl^13C/Ubx¹³⁰ siblings (6.3%). This increased transformation is often accompanied by a notal transformation. (Faucheux et al., 2003) lolal^k02512 mutants exhibit cuticular defects. The position of the first abdominal denticle belt is moved posteriorly, indicating head involution defects associated with altered mouth hooks. lolal^k02512 mutants exhibit dorsal closure defects, to varying degrees of severity, from a hole in the dorsal cuticle to the absence of closure. lolal^k02512 embryos lacking both maternal and zygotic contribution of lolal display cuticular defects compared to the wild-type. Embryos show incomplete head involution with many exhibiting defects in dorsal closure. In contrast, embryos derived from lolal^k02512/lolal^k02512;Scer\GAL4^da.G32, lolal^{Scer\UAS.T:Avic\GFP-EGFP}/+ rescued females crossed to wild-type males hatch and do not display cuticular defects. Heteroallelic lolal^311a/lolal^k02512 flies have wild-type wings. lolal^Δ11π mutants, in combination with lolal^k02512 exhibit ectopic sex comb teeth on meso- or metathoracic legs in about one-fifth of the male escapers. (Faucheux et al., 2003) Addition of lolal^+tBB, lolal^{Scer\UAS.T:Zzzz\FLAG} or lolal^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 where necessary, to ph-p⁴¹⁰, lolal^k02512 double mutants results in rescue of the enhancement of the ph-p⁴¹⁰ esc-like phenotype, with a reduction in the number of homeotic transformations of mesothoracic or metathoracic legs towards prothoracic legs and the mean number of sex comb teeth to a level similar to that in the double mutant ph-p⁴¹⁰, lolal^k02512. (Faucheux et al., 2003) The addition of lolal^k02512/+ to homozygous ph-p⁴¹⁰ animals shows a partial homeotic transformation of A4 to A5, A5 to A6, and A6 to A7. There is a strong reduction of the size of the 6th tergite. Sex combs are seen on the second and third legs. The addition of lolal^k02512/+ to heterozygous Asx^XF23 animals shows a partial homeotic transformation of A6 to A5 in the abdomen. The addition of lolal^k02512/+ to heterozygous Pc¹ animals produces crumbled wings with defective margin. The addition of lolal^k02512/+ to heterozygous mor¹ animals produces rough eyes. trg^unspecified/+, lolal^k02512/+ animals show development of a small additional tergite, indicating a transformation of A7 to A6. Trl^13C/+, lolal^k02512/+ animals show no enhancement or suppression of the lolal^k02512 phenotype. The addition of heterozygous osa^unspecified, trx^unspecified, ash1^B1, Psc¹ or Scm^R5-13 to lolal^k02512/+ animals shows enhancement or suppression. (Mishra et al., 2003) Lethality occurs during third instar larval or pupal stages. Mutants do not show any visible disc abnormalities. (Roch et al., 1998)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k02512 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10515 y¹ w^67c23; P{lacW}lolal^k02512/CyO
Kyoto	111150 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}lolal^k02512 /CyO y⁺
Linkouts
Comments
	Location 2R:13671802-13671803 confirmed by FlyBase alignment of dbGSS accession AQ034138 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P[w+mC=lacEW]lolalk02512 (Mishra et al., 2003) P{lacW}l(2)k02512^k02512 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}lolal^k02512 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Kerman et al., 2008, Dev. Biol. 320(1): 278--288 Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin. [FBrf0207321] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Faucheux et al., 2003, Molec. Cell. Biol. 23(4): 1181--1195 batman Interacts with Polycomb and trithorax Group Genes and Encodes a BTB/POZ Protein That Is Included in a Complex Containing GAGA Factor. [FBrf0155961] Mishra et al., 2003, Mech. Dev. 120(6): 681--689 Trl-GAGA directly interacts with lola like and both are part of the repressive complex of Polycomb group of genes. [FBrf0160797] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112 Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}lolalk02512 Insertion

Dmel\P{lacW}lolal^k02512 Insertion