A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}snRNA:U6atac:29Bk01105 Insertion

General Information
Symbol Dmel\P{lacW}snRNA:U6atac:29Bk01105 Species D. melanogaster
Name FlyBase ID FBti0006906
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, snRNA:U6atac:29B Viability / fertility
Causes allele(s) Ecol\lacZsnRNA:U6atac:29B-k01105, snRNA:U6atac:29Bk01105 Stock availability 2 publicly available
LINE ID l(2)k01105
Genomic Location
Chromosomal location 2L ( 29C1 ) Sequence location 2L:8,389,793..8,389,793 [+]
Map ( GBrowse ) detailed view FBti0024896 FBti0115484 FBti0044189 FBti0039857 FBti0028239 FBti0105012 FBti0074688 FBti0025220 FBti0006455 FBti0070971 FBti0104769 FBti0035833 FBti0047193 FBti0006906 FBti0114022 FBti0076343 FBti0124662_2
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:8,389,793..8,389,793 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
29C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
29C1-29C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025697
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
snRNA:U6atac:29B
(BDGP Project Members, 1994-1999, Pessa et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZsnRNA:U6atac:29B-k01105
(BDGP Project Members, 1994-1999)
snRNA:U6atac:29Bk01105
(BDGP Project Members, 1994-1999, Otake et al., 2002, Roch et al., 1998, Spradling et al., 1999, Pessa et al., 2010)
Lethality
References
lethal | recessive
(Spradling et al., 1999, Roch et al., 1998, Otake et al., 2002)
Sterility
References
hide Phenotype Manifest In
crystal cell
(Milchanowski et al., 2004)
imaginal disc
(Roch et al., 1998)
hide Detailed Description
Statement
Reference
The average number of crystal cells per embryo is reduced (to 10.8) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).
(Milchanowski et al., 2004)
Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are of normal size and shape.
(Roch et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k01105
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10492
y1 w67c23; P{lacW}snRNA:U6atac:29Bk01105/CyO
Kyoto
114341
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}snRNA:U6atac:29Bk01105 P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:8389792-8389793 determined by FlyBase alignment of dbGSS accession AQ025697 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k01105k01105
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}snRNA:U6atac:29Bk01105
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Pessa et al., 2010, PLoS ONE 5(10): e13215
Gene expression profiling of U12-type spliceosome mutant Drosophila reveals widespread changes in metabolic pathways. [FBrf0212091]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Milchanowski et al., 2004, Genetics 168(1): 325--339
Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297]
Otake et al., 2002, Molec. Cell 9(2): 439--446
The divergent U12-type spliceosome is required for pre-mRNA splicing and is essential for development in Drosophila. [FBrf0145116]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]