Dmel\P{lacW}ed^k01102 Insertion

General Information
Symbol	Dmel\P{lacW}ed^k01102	Species	D. melanogaster
Name		FlyBase ID	FBti0006909
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, ed	Viability / fertility
Causes allele(s)	Ecol\lacZ^ed-k01102, ed^k01102	Stock availability	2 publicly available
LINE ID	l(2)k01102
Genomic Location
Chromosomal location	2L ( 24D4 )	Sequence location	2L:4,031,769..4,031,769 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:4,031,769..4,031,769 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	24D4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	24D3-24D4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034173 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999, Bai et al., 2001) ed (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^ed-k01102 (BDGP Project Members, 1994-1999, Bai et al., 2001) ed^k01102 (BDGP Project Members, 1994-1999, Spencer and Cagan, 2003, Rawlins et al., 2003, Ahmed et al., 2003, Bai et al., 2001, Spradling et al., 1999, Swan et al., 2006, Swan et al., 2006, Dworkin and Gibson, 2006)

Lethality References lethal \| larval stage \| recessive (Spencer and Cagan, 2003) lethal \| pupal stage (Ahmed et al., 2003) lethal \| pupal stage \| recessive (Bai et al., 2001) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006) partially lethal - majority die \| pupal stage (Rawlins et al., 2003)
Sterility References
Phenotype Manifest In
	chemosensory ventral triple row & microchaeta \| ectopic (Rawlins et al., 2003) dorsal triple row & microchaeta \| ectopic (Rawlins et al., 2003) dorsocentral bristle \| ectopic (Rawlins et al., 2003) eye (Ahmed et al., 2003) photoreceptor cell R7 \| somatic clone \| supernumerary (Spencer and Cagan, 2003) photoreceptor cell R8 \| somatic clone \| supernumerary (Spencer and Cagan, 2003) photoreceptor cell R8 \| supernumerary (Rawlins et al., 2003, Spencer and Cagan, 2003) scutellar bristle \| ectopic (Rawlins et al., 2003) sex comb (Ahmed et al., 2003) wing (Dworkin and Gibson, 2006)
Detailed Description
Statement Reference Heterozygotes show a direct effect on the shape of the wing. (Dworkin and Gibson, 2006) ed^k01102/ed^m1 animals usually die as pupae, but adult escapers are sometimes produced in low density cultures. These escapers have mild defects in the leg; the legs have the correct number of segments, but the sex combs are malformed in males. They also have a rough eye phenotype. (Ahmed et al., 2003) Twinning of R8 photoreceptor cells is seen in ed^k01102/Df(2L)ed-dp animals. (Rawlins et al., 2003) ed^lH23/ed^k01102 flies have extra dorsocentral, scutellar and recurved wing margin bristles (chemosensory ventral triple row + dorsal triple row). ed^6.1/ed^k01102 and ed^k01102/Df(2L)ed-dp flies have extra dorsocentral and scutellar bristles. (Rawlins et al., 2003) ed^k01102/+ strongly enhances the Egfr^E1/+ phenotype, resulting in eyes that are approximately 1/3 the size of those of Egfr^E1/+ flies. argos^Δ7/+ mildly enhances the ed^k01102 phenotype; the number of ommatidia with multiple R8 cells is increased from 18% to 34% in the double mutant flies. argos^W11 enhances the ed^k01102 phenotype; the number of ommatidia with multiple R8 cells is increased from 21% to 46% in the double mutant flies. (Spencer and Cagan, 2003) Homozygotes are viable until the late third larval instar. Homozygous and ed^k01102/ed^slA12 eye discs contain ommatidia with multiple R8 cells. This phenotype is also seen in homozygous clones in mosaic eye discs. Ommatidia in homozygous clones in the eye sometimes contain extra R7 cells and in all cases where ommatidia contain extra R7 cells, extra R8 cells are also present. (Spencer and Cagan, 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k01102 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10490 y¹ w^67c23; P{lacW}ed^k01102/CyO
Kyoto	111134 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}ed^k01102 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:4031769-4031770 confirmed by FlyBase alignment of dbGSS accession AQ034173 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P(lacZ)ed^k01102 (Swan et al., 2006) P{lacW}ed^k01102 (FlyBase, 2005, FlyBase, 1992-, ) P{lacW}l(2)k01102^k01102 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 16 )
Research paper	Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431 Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945] Swan et al., 2006, EMBO J. 25(15): 3640--3651 Complex interaction of Drosophila GRIP PDZ domains and Echinoid during muscle morphogenesis. [FBrf0192056] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Ahmed et al., 2003, Development 130(25): 6295--6304 echinoid mutants exhibit neurogenic phenotypes and show synergistic interactions with the Notch signaling pathway. [FBrf0167515] Rawlins et al., 2003, Development 130(26): 6475--6484 Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta. [FBrf0167521] Rawlins et al., 2003, Development 130(16): 3715--3724 Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups. [FBrf0160869] Spencer and Cagan, 2003, Development 130(16): 3725--3733 Echinoid is essential for regulation of Egfr signaling and R8 formation during Drosophila eye development. [FBrf0160973] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Swan et al., 2006, EMBO J. 25(15): Supplementary Methods. [FBrf0198715]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}edk01102 Insertion

Dmel\P{lacW}ed^k01102 Insertion