A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}CG11329k00605a Insertion

General Information
Symbol Dmel\P{lacW}CG11329k00605a Species D. melanogaster
Name FlyBase ID FBti0006931
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) CG11329, Ecol\lacZ, l(2)k00605b Viability / fertility viable
Causes allele(s) CG11329k00605a, Ecol\lacZCG11329-k00605a Stock availability 2 publicly available
LINE ID l(2)k00605
Genomic Location
Chromosomal location 2L ( 26F7 ) Sequence location 2L:6,677,574..6,677,574 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0023297 FBti0055322 FBti0049313 FBti0047740 FBti0070514 FBti0026453 FBti0069844 FBti0024442 FBti0054458 FBti0069543 FBti0054720 FBti0143942 FBti0011059 FBti0006931 FBti0104804 FBti0108185 FBti0024753 FBti0112540 FBti0106339 FBti0110461 FBti0110079 FBti0071084 FBti0018792_2 FBti0104670 FBti0072543
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:6,677,574..6,677,574 [-]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
26F7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
27A1-27A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025688
 
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG11329
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
l(2)k00605b
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
CG11329k00605a
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Ecol\lacZCG11329-k00605a
(BDGP Project Members, 1994-1999)
Lethality
References
viable
(Kaufmann et al., 2002)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k00605
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10480
y1 w67c23; P{lacW}CG11329k00605a, l(2)k00605bk00605b/CyO
Kyoto
114339
yd2 w1118 P{ey-FLP.N}2; P{lacW}CG11329k00605a, l(2)k00605bk00605b P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:6677573-6677574 determined by FlyBase alignment of dbGSS accession AQ025688 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
Separable from: l(2)k00605bk00605b.
(Kaufmann et al., 2002)
Separable from l(2)k00605bk00605b.
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}CG11329k00605a
(Kaufmann et al., 2002, FlyBase, 2005, FlyBase, 1992-, )
P{lacW}k00605a
(FlyBase, 1992-)
P{lacW}l(2)k00605k00605
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Kaufmann et al., 2002, Neuron 34(1): 27--38
Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis. [FBrf0147174]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]