Dmel\P{lacW}Mad^k00237 Insertion

General Information
Symbol	Dmel\P{lacW}Mad^k00237	Species	D. melanogaster
Name		FlyBase ID	FBti0006935
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Mad	Viability / fertility
Causes allele(s)	Ecol\lacZ^Mad-k00237, Mad^k00237	Stock availability	2 publicly available
LINE ID	l(2)k00237
Genomic Location
Chromosomal location	2L ( 23D3 )	Sequence location	2L:3,159,608..3,159,608 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01	Controlled Vocabulary Terms embryonic/larval neuromuscular junction References Ball et al., 2010, Neuron 66(4): 536--549
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:3,159,608..3,159,608 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	23D3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	23D3-23D4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034169 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Mad (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Mad-k00237 (BDGP Project Members, 1994-1999) Mad^k00237 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dworkin and Gibson, 2006, Kaneko et al., 2011)
Sterility References
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage (Ball et al., 2010) leg \| distal \| heat sensitive (Galindo et al., 2002) leg \| heat sensitive (Galindo et al., 2002) NMJ bouton \| third instar larval stage (Merino et al., 2009, Ball et al., 2010) wing (Dworkin and Gibson, 2006)
Detailed Description
Statement Reference Expression of Mad[hs.PK] using heat shock partially rescues the lethality of Mad[k00237] mutants, with the rescue efficiency being increased with more frequent heat shocks. Expression of Mad[T312V.hs] using heat shock partially rescues the lethality of Mad[k00237] mutants, with the rescue efficiency being increased with more frequent heat shocks. The degree of rescue with Mad[T312V.hs] is more effective than that seen with Mad[hs.PK] under the same conditions. (Kaneko et al., 2011) Mad[1]/Mad[k00237] third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls. Homozygous Mad[k00237] third instar larvae show a reduced number of boutons per muscle surface area in muscle 4 compared to controls. (Ball et al., 2010) Expression of Mad[1]/Mad[k00237] suppresses the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[Scer\UAS.T:Hsap\MYC] is expressed in motor neurons under the control of Scer\GAL4[BG380], instead producing a reduction in bouton number similar to the phenotype seen in Mad[1]/Mad[k00237] third instar larvae. Expression of Mad[1]/Mad[k00237] fails to suppress the larval neuromuscular junction overgrowth and bouton number phenotypes seen when Rac1[V12.Scer\UAS] is expressed in motor neurons under the control of Scer\GAL4[BG380]. trio[S137203] does not enhance the reduction in bouton number phenotype seen in Mad[k00237] larval neuromuscular junctions. Expression of trio[Scer\UAS.cBa] in motor neurons under the control of Scer\GAL4[BG380] partially suppresses the reduced bouton number phenotype seen in Mad[1]/Mad[k00237] larval neuromuscular junctions. (Ball et al., 2010) Neuronal expression of Mad[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[unspecified] rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad[k00237] third instar larvae. Neuronal expression of Mad[S25A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[unspecified] partially rescues the reduction in the number of boutons per muscle surface area that is seen at the neuromuscular junction in Mad[k00237] third instar larvae. Expression of either Mad[Scer\UAS.T:Hsap\MYC] or Mad[S25A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[BG380] rescues the electrophysiological defects seen at the neuromuscular junction of Mad[k00237] third instar larvae. (Merino et al., 2009) The number of boutons per muscle surface area is reduced at the neuromuscular junction in Mad[k00237] third instar larvae. Homozygous third instar larvae show a severe reduction in excitatory junctional current amplitude and in quantal content at the neuromuscular junction compared to wild type. (Merino et al., 2009) Mad[k00237]/+ ; nmo[P1]/+ and Mad[k00237]/+ ; Df(3L)Exel6279/+ double heterozygous third instar larvae show a significant reduction in the number of boutons per muscle surface area at the neuromuscular junction, in contrast to any of the single heterozygotes. (Merino et al., 2009) Heterozygotes show a direct effect on the shape of the wing. (Dworkin and Gibson, 2006) Removal of Mad function before 72 hours after egg laying in Mad^apg/Mad^k00237 flies produces truncations of the proximal-distal axis in the legs, whereas removal of Mad function from 84 hours after egg laying until the end of development results in a normal proximal-distal organisation in the leg despite dorsal patterning defects. (Galindo et al., 2002) Mad^k00237/Mad^apg larvae transferred to 29^oC at the equivalent of 48 hours, 72 hours, 84 hours or 96 hours after egg laying develop at the same rate as wild-type siblings. (Galindo et al., 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k00237 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10474 y¹ w^67c23; P{lacW}Mad^k00237/CyO
Kyoto	111123 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Mad^k00237 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:3159608-3159609 confirmed by FlyBase alignment of dbGSS accession AQ034169 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)k00237^k00237 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}Mad^k00237 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Kaneko et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(27): 11127--11132 Smad inhibition by the Ste20 kinase Misshapen. [FBrf0214278] Ball et al., 2010, Neuron 66(4): 536--549 Retrograde BMP Signaling Controls Synaptic Growth at the NMJ by Regulating Trio Expression in Motor Neurons. [FBrf0210920] Merino et al., 2009, J. Cell Biol. 185(4): 713--725 Nemo kinase interacts with Mad to coordinate synaptic growth at the Drosophila neuromuscular junction. [FBrf0208030] Dworkin and Gibson, 2006, Genetics 173(3): 1417--1431 Epidermal growth factor receptor and transforming growth factor-beta signaling contributes to variation for wing shape in Drosophila melanogaster. [FBrf0193945] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Galindo et al., 2002, Science 297(5579): 256--259 Leg patterning driven by proximal-distal interactions and EGFR signaling. [FBrf0151488] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Galindo et al., 2002, Science 296(5579): Supplementary material: leg patterning driven by proximal-distal interactions and EGFR signaling. [FBrf0157204]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Madk00237 Insertion

Dmel\P{lacW}Mad^k00237 Insertion