A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Madk00223 Insertion

General Information
Symbol Dmel\P{lacW}Madk00223 Species D. melanogaster
Name FlyBase ID FBti0006936
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, gkt, Mad Viability / fertility
Causes allele(s) Ecol\lacZMad-k00223, gktk00223, Madk00223 Stock availability none publicly available
LINE ID l(2)k00223
Genomic Location
Chromosomal location 2L ( 23D3 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
23D3 ( near gene of known cytology )
Cytological location
(reported)
23D3-23D4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
gkt
(Dunlop et al., 2004)
Mad
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZMad-k00223
(BDGP Project Members, 1994-1999)
gktk00223
(Dunlop et al., 2004)
Madk00223
(BDGP Project Members, 1994-1999, Dunlop et al., 2000, Dunlop et al., 2004, Roch et al., 1998)
Lethality
References
lethal
(Dunlop et al., 2004)
lethal | recessive
(Roch et al., 1998)
Sterility
References
hide Phenotype Manifest In
imaginal disc
(Roch et al., 1998)
hide Detailed Description
Statement
Reference
Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: discs are small. Clones induced in wild type discs are abnormal: altering cell differentiation.
(Roch et al., 1998)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k00223
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
l(2)k00223
(Dunlop et al., 2004, FlyBase, 1992-)
P{lacW}l(2)k00237k00223
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Madk00223
(FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Dunlop et al., 2004, Curr. Biol. 14(22): 2039--2045
glaikit is essential for the formation of epithelial polarity and neuronal development. [FBrf0180073]
Dunlop et al., 2000, Mech. Dev. 96(1): 133--136
The novel gene glaikit, is expressed during neurogenesis in the Drosophila melanogaster embryo. [FBrf0129794]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Roch et al., 1998, Mol. Gen. Genet. 257(2): 103--112
Screening of larval/pupal P-element induced lethals on the second chromosome in Drosophila melanogaster: clonal analysis and morphology of imaginal discs. [FBrf0100624]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]