A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Dmel\P{lacW}Sec61βk07836 Insertion

General Information
Symbol Dmel\P{lacW}Sec61βk07836 Species D. melanogaster
Name FlyBase ID FBti0006961
Feature type transposable_element_insertion_site Created / Updated 1998-01-03/2000-05-31
Description
Inserted element P{lacW} Localized function
Affected gene(s) Sec61β Expression data
Causes allele(s) Sec61βk07836 Viability / fertility
LINE ID l(2)k07836 Stock availability
Genomic Location
Chromosomal location 2R ( 51B6 ) Sequence location
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
51B6 ( near gene of known cytology )
Cytological location
(reported)
51B7-51B8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Chromosome in situ
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role 		 
Size 10.691Kb
Associated alleles
w+mC
Ecol\lacZP\T.W
Molecular map
Transposon class
Element type
hide Affected Gene(s)
Insertion within gene
Insertion may
affect gene
Sec61β
(BDGP Project Members, 1994-1999)
Orientation
(relative to gene)
Gene
Orientation
References
 
 
 
hide Alleles and Phenotypes
Causes alleles
Sec61βk07836
(BDGP Project Members, 1994-1999, Valcarcel et al., 1999)
Lethality
References
lethal | recessive
(Valcarcel et al., 1999, BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
embryonic/first instar larval cuticle
(Valcarcel et al., 1999)
leg
(Valcarcel et al., 1999)
hide Detailed Description
Statement
Reference
Homozygous embryos have similar cuticle defects to Sec61β14448 homozygotes. Sec61β07214/Sec61βk07836 escapers show defects in leg development.
(Valcarcel et al., 1999)
Lethality is rescued by Sec61βTub.PV.
(Valcarcel et al., 1999)
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k07836
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)07214k07836
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}Sec61βk07836
(FlyBase, 1992-)
Secondary FlyBase IDs
    hide References ( 6 )
    Research paper
    Bellen et al., 2004, Genetics 167(2): 761--781
    The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
    Spradling et al., 1999, Genetics 153(1): 135--177
    The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
    Valcarcel et al., 1999, J. Cell Sci. 112(23): 4389--4396
    Sec61(), a subunit of the protein translocation channel, is required during Drosophila development. [FBrf0112063]
    FlyBase analysis
    FlyBase, 1992-, FlyBase curation
    FlyBase curation. [FBrf0105495]
    Computer file
    Gene Disruption Project members, 2001-, [title not yet available]
    [title not yet available] [FBrf0132177]
    BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
    Berkeley Drosophila Genome Project. [FBrf0067338]