Dmel\P{lacW}capt^k01217 Insertion

General Information
Symbol	Dmel\P{lacW}capt^k01217	Species	D. melanogaster
Name		FlyBase ID	FBti0006967
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	capt, Ecol\lacZ	Viability / fertility
Causes allele(s)	capt^k01217, Ecol\lacZ^capt-k01217	Stock availability	2 publicly available
LINE ID	l(2)k01217
Genomic Location
Chromosomal location	2L ( 21F1 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	21F1 ( near gene of known cytology )
Cytological location (reported)	21F1-21F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	capt (BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	capt^k01217 (BDGP Project Members, 1994-1999, Benlali et al., 2000, Baum, 1999.9.26, Spradling et al., 1999) Ecol\lacZ^capt-k01217 (BDGP Project Members, 1994-1999)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	oocyte & actin filament \| ectopic \| germ-line clone (Baum et al., 2000)
Detailed Description
Statement Reference Central nervous system axons are seen to cross the midline in Abl² capt^k01217 double heterozygous embryos. The frequency of ectopic crossing of the midline by axons in the central nervous system seen in sli² heterozygous embryos is increased if they are also heterozygous for capt^k01217. One copy of capt^k01217 enhances the frequency of midline crossing defects seen in robo¹ lea^robo2-8 double mutant heterozygotes. capt^k01217/Df(2L)ast2 embryos show ectopic midline crossing by axons in the central nervous system. Midline glia are still present in these embryos. (Wills et al., 2002) The combination of capt^k01217 and Pka-R1^BDK.Scer\UAS in germ-line clones produces an exaggerated phenotype, with complete fusion of the germline and ectopic actin aggregates at the posterior pole of the syncytium. (Baum et al., 2000) In capt^k01217 germ-line clones, ectopic F-actin cables form in the oocyte at stages 5-6 of oogenesis, only to disappear at stages 8-9. Actin aggregates later at the cortex of capt mutant eggs. (Baum et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k01217 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10367 y¹ w^67c23; P{lacW}capt^k01217/CyO
Kyoto	111068 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}capt^k01217 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}capt^k01217 (FlyBase, 1992-) P{lacW}capulet^k01217 (FlyBase, 1992-) P{lacW}l(2)06955^k01217 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Wills et al., 2002, Neuron 36(4): 611--622 A Drosophila homolog of cyclase-associated proteins collaborates with the abl tyrosine kinase to control midline axon pathfinding. [FBrf0152273] Baum et al., 2000, Curr. Biol. 10(16): 964--973 A cyclase-associated protein regulates actin and cell polarity during Drosophila oogenesis and in yeast. [FBrf0129724] Benlali et al., 2000, Cell 101(3): 271--281 act up controls actin polymerization to alter cell shape and restrict Hedgehog signaling in the Drosophila eye disc. [FBrf0128396] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Baum, 1999.9.26, EDRC99 - abstract ID200. EDRC99 - abstract ID200. [FBrf0111601]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}captk01217 Insertion

Dmel\P{lacW}capt^k01217 Insertion