Dmel\P{lacW}rig^k07917 Insertion

General Information
Symbol	Dmel\P{lacW}rig^k07917	Species	D. melanogaster
Name		FlyBase ID	FBti0006992
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	bl, Ecol\lacZ, rig	Viability / fertility
Causes allele(s)	Ecol\lacZ^rig-k07917, rig^k07917	Stock availability	2 publicly available
LINE ID	l(2)k07917
Genomic Location
Chromosomal location	2R ( 57A8 )	Sequence location	2R:16,528,148..16,528,148 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:16,528,148..16,528,148 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	57A8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	57A5-57A6 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ034008 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	bl (BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999) rig (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^rig-k07917 (BDGP Project Members, 1994-1999) rig^k07917 (BDGP Project Members, 1994-1999, Spradling et al., 1999)

Lethality References lethal \| prepupal stage (Gates et al., 2004) lethal \| pupal stage (Gates et al., 2004) lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999) semi-lethal \| larval stage (Gates et al., 2004) semi-lethal \| prepupal stage (Gates et al., 2004)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference There first stage at which significant lethality of rig^k07917/Df(2R)exu1 or rig^k07917/rig^k07839 animals occurs is the 2nd instar. Of those mutants that die as second instar larvae approximately half survive two or more days past the time when they should have molted to the third instar and obtain the size of a late third instar, but retain the anterior spiracles of a second instar larva. These larvae usually become stationary and die around the same time as their third instar siblings. Some mutants enter the 2nd to 3rd instar molt, but die during this process. Such larvae have two sets of mouth parts and, in some cases, two sets of anterior spiracles and/or two cuticles. Of those mutant animals that do survive the molt, approximately half stop moving during the first day of the third instar, becoming extended and stiff in an apparent attempt to pupariate. They survive in this immobile state for 2-3 days, after which patches of necrotic cells form throughout the animal. Most of the remaining mutant third instar larvae become stationary over the next few days, while some stop eating and shrink in size until they finally die. Some of the mutants pupariate normally, although 1-5 days later than control animals, and then die as prepupae. A few rig^k07917/Df(2R)exu1 animals (but no rig^k07917/rig^k07839 animals) make it to the pupal stage before dying. (Gates et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k07917 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10691 y¹ w^67c23; P{lacW}rig^k07917/CyO
Kyoto	111229 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}rig^k07917 /CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}bl^k07917 (FlyBase, 1992-) P{lacW}l(2)05056^k07917 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}rig^k07917 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Gates et al., 2004, Development 131(1): 25--36 rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development. [FBrf0167527] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}rigk07917 Insertion

Dmel\P{lacW}rig^k07917 Insertion