Dmel\P{lacW}crol^k05205 Insertion

General Information
Symbol	Dmel\P{lacW}crol^k05205	Species	D. melanogaster
Name		FlyBase ID	FBti0007010
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	crol, Ecol\lacZ	Viability / fertility
Causes allele(s)	crol^k05205, Ecol\lacZ^crol-k05205	Stock availability	2 publicly available
LINE ID	l(2)k05205 l(2)05205
Genomic Location
Chromosomal location	2L ( 33A1-33A2 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	33A1-33A2 ( near gene of known cytology )
Cytological location (reported)	33A1-33A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	crol (BDGP Project Members, 1994-1999) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	crol^k05205 (BDGP Project Members, 1994-1999, Spradling et al., 1999) Ecol\lacZ^crol-k05205 (BDGP Project Members, 1994-1999)

Lethality References lethal \| recessive (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In
	wing pouch \| somatic clone (Mitchell et al., 2008)
Detailed Description
Statement Reference crol[k05205]/+ enhances the reduced wing phenotype caused by expression of pUf68[Scer\UAS.cQa] under the control of Scer\GAL4[en-e16E] in the posterior compartment. Analysis of 120 hour larval wing discs indicates that the growth of crol[k05205] clones is restored if they are induced in a arm[4]/+ background compared to when they are induced in an otherwise wild-type background. (Mitchell et al., 2008) Homozygous clones are rarely seen in the wing pouch of wing discs from 120 hour larvae (clones induced at 72 hours after egg deposition), in contrast to control clones. Homozygous mutant clones are recovered in the hinge region of these discs. Small homozygous clones are recovered in the wing pouch in 96 hour larval wing discs (clones induced at 48 hours after egg deposition). Clonal cells with a pyknotic morphology are seen in the basal section of the wing pouch in these discs. The homozygous cells show reduced BrdU incorporation and a reduction in the number of mitotic cells compared to wild-type clones. (Mitchell et al., 2008) Co-expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[tub] in homozygous crol[k05205] clones results in more mutant cells being seen in the wing pouch of wing discs from 120 hour larvae (clones induced at 72 hours after egg deposition) compared to homozygous crol[k05205] clones induced in an otherwise wild-type background. (Mitchell et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)05205 (Gene Disruption Project members, 2001-) l(2)k05205 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line	t (Gene Disruption Project members, 2001-)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10386 y¹ w^67c23; P{lacW}crol^k05205/CyO
Kyoto	111079 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}crol^k05205 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(2)05205 (FlyBase, 1992-) P{lacW}crol^k05205 (FlyBase, 1992-) P{lacW}l(2)04418^k05205 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Mitchell et al., 2008, Development 135(16): 2707--2716 The Ecdysone-inducible zinc-finger transcription factor Crol regulates Wg transcription and cell cycle progression in Drosophila. [FBrf0207008] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Chu et al., 2001, genesis 29(3): 141--152 Cortex, a Drosophila gene required to complete oocyte meiosis, is a member of the Cdc20/fizzy protein family. [FBrf0134862] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}crolk05205 Insertion

Dmel\P{lacW}crol^k05205 Insertion