Dmel\P{lacW}tutl^k14703 Insertion

General Information
Symbol	Dmel\P{lacW}tutl^k14703	Species	D. melanogaster
Name		FlyBase ID	FBti0007070
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, tutl	Viability / fertility
Causes allele(s)	Ecol\lacZ^tutl-k14703, tutl^k14703	Stock availability	2 publicly available
LINE ID	l(2)k14703
Genomic Location
Chromosomal location	2L ( 24E1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	24E1 ( near gene of known cytology )
Cytological location (reported)	24E1-24E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) tutl (BDGP Project Members, 1994-1999, Al-Anzi and Wyman, 2009, Ferguson et al., 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^tutl-k14703 (BDGP Project Members, 1994-1999) tutl^k14703 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Dean, 2003.6.5, Bodily et al., 2001, Al-Anzi and Wyman, 2009, Ferguson et al., 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009)

Lethality References lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999) partially lethal - majority die \| pupal stage (Al-Anzi and Wyman, 2009) partially lethal - majority live (Bodily et al., 2001)
Sterility References
Phenotype Manifest In
	intersegmental nerve branch ISNb of A1-7 (Al-Anzi and Wyman, 2009) intersegmental nerve branch ISNd of A1-7 (Al-Anzi and Wyman, 2009) optic chiasm (Al-Anzi and Wyman, 2009) photoreceptor cell R7 (Ferguson et al., 2009)
Detailed Description
Statement Reference Expression of either tutl[GH15753.Scer\UAS] or tutl[AT02763.Scer\UAS] driven by the pan-neuronal driver Scer\GAL4[elav-C155] rescues most aspects of tutl[k14703]/tutl[ex383] mutant eye defects. (Al-Anzi and Wyman, 2009) In tutl[k14703] embryos, the ISNb motor axons succesfully reach the vicinity of their respective targets. However, once there, many fail to send one or more of the final axon branches to contact their muscle targets. Around a quarter of the hemisegments also lack ISNd nerves. Approximately 20% of tutl[k14703] homozygotes reach adulthood. They exhibit an optic chiasm that is disorganised and exhibits graps in the R7 termination line. Flies with eyes composed of tutl[k14703] mutant cells and brains expressing wild-type levels of tutl (generated using the EGUF technique) exhibit smaller eyes than wild-type, although the optic chiasma and R7 projections appear normal, indicating that the source of retinal axon defects in tutl[k14703] mutants is reduced tutl levels in the optic lobe, rather than in the retinal axons. (Al-Anzi and Wyman, 2009) Expression of tutl[Scer\UAS.cFa] under the control of Scer\GAL4[GMR.long] rescues the mild tiling phenotype found in tutl[k14703]/tutl[23] R7 terminals. (Ferguson et al., 2009) tutl[k14703]/tutl[23] mutants display a mild R7 tiling phenotype. (Ferguson et al., 2009) Homozygous tutl[k14703] embryos show no gastrulation defects. (Mathew et al., 2009) A tutl[k14703] ; tutl[01085] mutant background enhances the moderately rough eye phenotype observed upon expression of mbl[C.Scer\UAS] under the control of Scer\GAL4[hs.2sev]. (Vicente-Crespo et al., 2008) Most homozygotes are able to survive to adulthood if competition with heterozygous siblings is eliminated. However, competition from heterozygous siblings results in the death of homozygous animals (85-100% lethality) before adulthood. General morphology of the mutant larvae is normal. Homozygous larvae have a severely compromised ability to roll over from an inverted position; the time required to right themselves is significantly longer than control larvae. Homozygous adults cannot fly, although they walk normally and energetically on a level surface. The jump response is intact and robust, but attempts at flight result in the flies flipping themselves onto their backs, where continued efforts to fly are shown by the adults flapping their wings at high speeds (this only results in the flies spinning frantically in circles). Adults that become inverted are rarely able to right themselves, despite what appears to be an intense effort to roll over. Only 12-36% of adults are able to roll over after 60 seconds, in contrast to wild-type which roll over immediately, almost always in less than one second. (Bodily et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k14703 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10451 y¹ w^67c23; P{lacW}tutl^k14703/CyO
Kyoto	111106 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}tutl^k14703 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(2)01085^k14703 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}tutl^k14703 (FlyBase, 1992-, ) tutl^k14703 (Al-Anzi and Wyman, 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009) tutl^K14703 (Ferguson et al., 2009)
Secondary FlyBase IDs

References ( 13 )
Research paper	Al-Anzi and Wyman, 2009, Neural Dev. 4: 31 The Drosophila immunoglobulin gene turtle encodes guidance molecules involved in axon pathfinding. [FBrf0209104] Ferguson et al., 2009, J. Neurosci. 29(45): 14151--14159 The conserved Ig superfamily member turtle mediates axonal tiling in Drosophila. [FBrf0209317] Mathew et al., 2009, PLoS ONE 4(10): e7437 A small genomic region containing several loci required for gastrulation in Drosophila. [FBrf0208913] Vicente-Crespo et al., 2008, PLoS ONE 3(2): e1613 Drosophila muscleblind is involved in troponin T alternative splicing and apoptosis. [FBrf0210274] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Bodily et al., 2001, J. Neurosci. 21(9): 3113--3125 A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control. [FBrf0135975] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Dean, 2003.6.5, Noncomplementation data. Noncomplementation data. [FBrf0159868] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}tutlk14703 Insertion

Dmel\P{lacW}tutl^k14703 Insertion