A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}wechk08815a Insertion

General Information
Symbol Dmel\P{lacW}wechk08815a Species D. melanogaster
Name FlyBase ID FBti0007097
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) dpld, Ecol\lacZ, wech Viability / fertility
Causes allele(s) Ecol\lacZk08815a, wechk08815a Stock availability 3 publicly available
LINE ID l(2)k08815
Genomic Location
Chromosomal location 2R ( 43C5 ) Sequence location 2R:3,377,339..3,377,339 [-]
Map ( GBrowse ) detailed view FBti0112948 FBti0106470 FBti0106465 FBti0104055 FBti0110752 FBti0111555 FBti0105340 FBti0024709 FBti0107793 FBti0029039 FBti0045144 FBti0035279 FBti0026351 FBti0007758 FBti0011010 FBti0010727 FBti0103472 FBti0021119 FBti0007097 FBti0037213 FBti0130045 FBti0113077 FBti0076952 FBti0112894 FBti0028787 FBti0059062 FBti0102618 FBti0028527 FBti0057871 FBti0034346 FBti0030165 FBti0125004 FBti0050437 FBti0070513 FBti0068549 FBti0057844 FBti0036900 FBti0100826 FBti0057906 FBti0029232 FBti0059557 FBti0099987 FBti0021787 FBti0037453 FBti0054008 FBti0029484 FBti0027038 FBti0036498 FBti0125005_1 FBti0125005_2 FBti0026203 FBti0072215
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2012_01
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:3,377,339..3,377,339 [-]
(FlyBase, 2007.6)
Orientation
Cytological location
(computed by FlyBase)
43C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
43D1-43D4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025871
(FlyBase, 1992-, BDGP Project Members, 1994-1999, FlyBase, 2007.6)
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
dpld
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(BDGP Project Members, 1994-1999)
wech
(Loer et al., 2008)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZk08815a
(BDGP Project Members, 1994-1999)
wechk08815a
(Loer et al., 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k08815
(Gene Disruption Project members, 2001-, FlyBase, 2007.6)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 3 )
Bloomington
10818
y1 w67c23; P{lacW}wechk08815a P{lacW}k08815b, dpldk08815/CyO
Kyoto
102670
y1 w67c23 ; P{lacW}wechk08815a / CyO
111249
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}wechk08815a P{lacW}k08815b /CyO y+
hide Linkouts
hide Comments
There are are two flanking sequences for the "k08815" line; AQ025871, which maps to within the CG1624 transcription unit, and AQ034113, which maps just upstream of the CG2064 transcription unit. This suggests that there may be two insertions in the "k08815" line. The information in FBrf0204992 indicates that the insertion in CG1624 (represented by AQ025871, FBti0007097) does not cause the "dappled" mutant phenotype of the "k08815" line. There is no information as to whether the insertion upstream of CG2064 (represented by AQ034113, FBti0025321) causes the "dappled" mutant phenotype. Thus, a separate allele (dpldk08815, FBal0064902), not linked to either insertion, has been created in FlyBase to accommodate the "dappled" mutant phenotype of the "k08815" line.
(FlyBase, 1996-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}dpldk08815
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}dpldk08815a
(FlyBase, 1992-)
P{lacW}k08815a
(FlyBase, 1992-)
P{lacW}wechk08815a
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Loer et al., 2008, Nat. Cell Biol. 10(4):
Supplementary information. [FBrf0204992]
FlyBase analysis
FlyBase, 2007.6, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0199566]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]