A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{lacW}U2af38k14504 Insertion

General Information
Symbol Dmel\P{lacW}U2af38k14504 Species D. melanogaster
Name FlyBase ID FBti0007108
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Hop, U2af38 Viability / fertility
Causes allele(s) Ecol\lacZU2af38-k14504, Hopk14504, U2af38k14504 Stock availability 2 publicly available
LINE ID l(2)k14504
Genomic Location
Chromosomal location 2L ( 21B8 ) Sequence location 2L:294,632..294,632 [+]
Map ( GBrowse ) detailed view FBti0057573 FBti0100811 FBti0007603 FBti0055458 FBti0023465 FBti0030160 FBti0033668 FBti0044567 FBti0039404 FBti0053045 FBti0034914 FBti0055237 FBti0057807 FBti0028662 FBti0100378 FBti0035463 FBti0072540 FBti0025868 FBti0109017 FBti0007108 FBti0108471 FBti0112995 FBti0005315 FBti0124479_2 FBti0124479_1 FBti0065042 FBti0043805 FBti0010706 FBti0100111 FBti0052028 FBti0046854 FBti0039328 FBti0067787 FBti0047990 FBti0108727
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Description
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This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:294,632..294,632 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
21B8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
21B7-21B8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026079
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Hop
(Weinkove et al., 1999, Deal and Cook, 2001.4.24)
U2af38
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZU2af38-k14504
(BDGP Project Members, 1994-1999)
Hopk14504
(Weinkove et al., 1999, Deal and Cook, 2001.4.24)
U2af38k14504
(BDGP Project Members, 1994-1999, Spradling et al., 1999, Weinkove et al., 1999, Deal and Cook, 2005.3.10, Deal and Cook, 2001.4.24)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
crystal cell
(Milchanowski et al., 2004)
hide Detailed Description
Statement
Reference
The average number of crystal cells per embryo is reduced (to 12.5) in homozygous stage 13-14 embryos compared to wild type (36 +/- 2.2 cells on average).
(Milchanowski et al., 2004)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k14504
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10448
y1 w67c23; P{lacW}U2af38k14504, Hopk14504/CyO
Kyoto
111104
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}U2af38k14504, Hopk14504 P{neoFRT}40A/CyO y+
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hide Comments
Location 2L:294632-294633 confirmed by FlyBase alignment of dbGSS accession AQ026079 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}U2af38k14504
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Milchanowski et al., 2004, Genetics 168(1): 325--339
Identification and characterization of genes involved in embryonic crystal cell formation during Drosophila hematopoiesis. [FBrf0180297]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Weinkove et al., 1999, Curr. Biol. 9(18): 1019--1029
Regulation of imaginal disc cell size, cell number and organ size by Drosophila class I(A) phosphoinositide 3-kinase and its adaptor. [FBrf0111517]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Deal and Cook, 2005.3.10, Isolation and characterization of Df(2L)BSC106.
Isolation and characterization of Df(2L)BSC106. [FBrf0182721]
Deal and Cook, 2001.4.24, Isolation and Characterization of Df(2L)BSC4.
Isolation and Characterization of Df(2L)BSC4. [FBrf0136028]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]