A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}argEP452 Insertion

General Information
Symbol Dmel\P{EP}argEP452 Species D. melanogaster
Name FlyBase ID FBti0007317
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) arg, elav Viability / fertility viable
Causes allele(s) argEP452 Stock availability none publicly available
LINE ID EP(X)0452
Genomic Location
Chromosomal location X ( 1B8 ) Sequence location X:417,309..417,309 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0015667 FBti0015690 FBti0048930 FBti0013309 FBti0053754 FBti0066799 FBti0055391 FBti0059417 FBti0055580 FBti0069614 FBti0037307 FBti0039579 FBti0007373 FBti0007317 FBti0068633 FBti0030334 FBti0023123 FBti0034905 FBti0111226 FBti0053914 FBti0029719 FBti0059393 FBti0029973 FBti0029896 FBti0033993 FBti0033758 FBti0035206 FBti0027947 FBti0002575 FBti0070612 FBti0072036 FBti0109069 FBti0068706 FBti0069179 FBti0035022 FBti0099879 FBti0030211 FBti0013429 FBti0110061 FBti0071928 FBti0042002 FBti0076078 FBti0128257
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:417,309..417,309 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
1B8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
1B4-1B4 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
1B7-1B8 (in situ hybridization reported)
(Spradling, 1997.8.22)
1B7-1B8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025344
(Benos et al., 2001, FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
arg
(BDGP Project Members, 2000-, Benos et al., 2001)
elav
(Benos et al., 2001)
hide Alleles and Phenotypes
Causes alleles
argEP452
(Benos et al., 2001, Gene Disruption Project members, 2001-)
Lethality
References
viable
(Spradling, 1997.8.22)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)0452
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location X:380098-380099 confirmed by FlyBase alignment of dbGSS accession AQ025344 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
Maps to clone: 65F1
(Benos et al., 2001)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(1)0452
(Benos et al., 2001)
ep(x)0452
(FlyBase, 1992-)
EP(X)0452
(FlyBase, 1992-)
P{EP}argEP452
(FlyBase, 2005, FlyBase, 1992-, )
P{EP}EP452
(FlyBase, 1992-)
P{w}EP452
(Spradling, 1997.8.22)
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Benos et al., 2001, Genome Res. 11(5): 710--730
From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Spradling, 1997.8.22, EP lines from BDGP.
EP lines from BDGP. [FBrf0100829]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]